Anti-TNF-alpha Antibody

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Anti-TNF-alpha Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-TNF-alpha Antibody
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Human
特异性
Human TNF-alpha
免疫原
Recombinant Human TNF-alpha / TNFa / TNFSF2 protein (Catalog#10602-HNAE)
制备方法
Produced in rabbits immunized with purified, recombinant Human TNF-alpha / TNFa / TNFSF2 (rh TNF-alpha / TNFa / TNFSF2; Catalog#10602-HNAE; NP_000585.2; Val77-Leu233). TNF-alpha / TNFa / TNFSF2 specific IgG was purified by Human TNF-alpha / TNFa / TNFSF2 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-TNF-alpha Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-TNF-alpha Antibody (Rabbit Polyclonal 抗体) 图片

TNFa was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

Lane C:0.5 mg SW480 Whole Cell Lysate

2 µL anti-TNFa rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-TNFa rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 23 kDa

Observed band size: 33 kDa

Anti-TNF-alpha rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:25 kDa

Observed band size:27 kDa

Anti-TNF-alpha Antibody: 别称

Anti-DIF Antibody; Anti-TNF-alpha Antibody; Anti-TNFA Antibody; Anti-TNFSF2 Antibody

TNF-alpha 背景信息

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD12a; p55/6) and TNF-R2 (TNF receptor type 2; CD12b; p75/8), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
全称
tumor necrosis factor
研究领域
参考文献
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1
    Author
    Jiao, X;Yu, W;Qian, J;Chen, Y;Wei, P;Fang, W;Yu, G;
    Year
    2018
    Journal
    BMC Cancer
    Application
    WB
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