pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|大鼠 TPPP3 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80727-ACG|
|大鼠 TPPP3 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80727-ACR|
|大鼠 TPPP3 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||RG80727-ANG|
|大鼠 TPPP3 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||RG80727-ANR|
|大鼠 TPPP3 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80727-CF|
|大鼠 TPPP3 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80727-CH|
|大鼠 TPPP3 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80727-CM|
|大鼠 TPPP3 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80727-CY|
|大鼠 TPPP3 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80727-NF|
|大鼠 TPPP3 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80727-NH|
|大鼠 TPPP3 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80727-NM|
|大鼠 TPPP3 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80727-NY|
|大鼠 TPPP3 基因ORF全长cDNA(克隆载体)||RG80727-U|
|大鼠 TPPP3 基因ORF全长cDNA克隆(表达载体)||RG80727-UT|
TPPP3, a member of the Tubulin polymerization-promoting protein family, is an intrinsically unstructured protein that induces tubulin polymerization. TPPP3 is a marker in the developing musculoskeletal system. In tendons, Tppp3 is expressed in cells at the circumference of the developing tendons, likely the progenitors of connective tissues that surround tendons: the tendon sheath, epitenon, and paratenon. Tppp3 is also expressed in forming synovial joints. The onset of Tppp3 expression in joints coincides with cavitation, representing a molecular marker that can be used to indicate this stage in joint transition in joint differentiation. In late embryonic stages, Tppp3 expression highlights other demarcation lines that surround differentiating tissues in the forelimb.
Depletion of TPPP3 by microRNA-based RNA interference (RNAi) inhibits cell growth, arrests cell cycles, and causes mitotic abnormalities in HeLa cells. C57BL/6 mice that received subcutaneously injected LLC (Lewis lung carcinoma) cells in which TPPP3 was knocked down showed a pronounced reduction in tumor progression. The migration/invasion activity of TPPP3-knockdown LLC cells was significantly suppressed in a transwell chamber migration assay. When these cells were injected into the tail veins of C57BL/6 mice, they exhibited milder lung metastasis compared with control tumor cells. Taken together, these findings suggested that the TPPP3 gene played an important role in tumorigenesis and metastasis, and it could potentially become a novel target for cancer therapy.