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小鼠 TSC22D3 基因全长ORF克隆

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Mouse TSC22D3 cDNA Clone产品信息
NCBI注册码:
参考序列ORF长度:
cDNA基因描述:
分子别称:
分子种属:
载体:
质粒:
限制性酶切位点:
Tag序列:
序列信息:
测序引物:
启动子:
Application:
抗生素(大肠杆菌):
抗生素(哺乳动物细胞):
运输方式:
储存方法:
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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