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人 TWF1 基因全长ORF克隆 (表达载体), 无标签

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Human TWF1 cDNA Clone产品信息
NCBI注册码:
参考序列ORF长度:
cDNA基因描述:
分子别称:
分子种属:
载体:
限制性酶切位点:
Tag序列:
序列信息:
运输方式:
储存方法:
Human TWF1 基因质粒图
Human TWF1 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
pCMV/hygro Vector Information
 
Vector Name pCMV/hygro
Vector Size 5657bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag None
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)


Schematic of pCMV/hygro Multiple Cloning Sites
Product nameProduct name
研究背景

Twinfilin-1, also known as Protein A6, Protein tyrosine kinase 9, TWF1 and PTK9, is a cytoplasm protein which belongs to the actin-binding proteins ADF family and Twinfilin subfamily. Twinfilin-1 (TWF1 / PTK9 ) is a highly conserved actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. In addition to the mammalian twinfilin-1, a second protein with approximately 65% sequence identity to twinfilin-1 exists in mouse and humans. TWF1 / PTK9 is expressed at high levels in the colon, testis, ovary, prostate and lung. It is expressed at lower levels in the brain, bladder and heart. It is not detected in liver. TWF1 / PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. TWF1 / PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

参考资料
  • Beeler JF, et al.,1994, Mol Cell Biol 14 (2): 982-8.
  • Palmgren S, et al., 2002, J. Cell. Sci. 115 (Pt 5): 881-6.
  • Vartiainen MK, et al.,2003, J. Biol. Chem. 278 (36): 34347-55.
  • Hassel S, et al.,2004, Proteomics 4 (5): 1346-58.
  • Moseley,J.B. et al., 2006, J Cell Sci. 119 (Pt 8):1547-57.
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