Anti-TIE2 Antibody

Cat: 10700-R116

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Anti-TIE2 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-TIE2 Antibody
Validated applications
ELISA,ELISA(Cap),FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human TIE2
Immunogen
Recombinant Human Tie2 / CD202b / TEK protein (Catalog#10700-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Tie2 / CD202b / TEK (rh Tie2 / CD202b / TEK; Catalog#10700-H08H; NP_000450.2; Met1-Lys745).
Source
Monoclonal Rabbit IgG Clone #116
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-TIE2 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution Notes
ELISA 1:5000-1:10000  
ICC-IF 1:20-1:100  
FCM 1:25-1:100  
ELISA(Cap) 1:250-1:2000 This antibody will detect Human Tie2 / CD202b / TEK in ELISA pair set (Catalog: # SEK10700). In a sandwich ELISA, it can be used as capture antibody.

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-TIE2 Antibody (Rabbit Monoclonal antibody) Images

Human Tie2/CD202b/TEK Immunofluorescence(IF) 2850
Immunofluorescence staining of Human Tie2 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Tie2 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.
Human Tie2/CD202b/TEK Flow Cytometry (FC) 5012
Flow cytometric analysis of Human TIE2(CD202b) expression on HUVEC cells. Cells were stained with purified anti-Human TIE2(CD202b), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-TIE2 Antibody Alternative Names

Anti-CD202B Antibody;Anti-TIE-2 Antibody;Anti-TIE2 Antibody;Anti-VMCM Antibody;Anti-VMCM1 Antibody

TIE2 Background Information

TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Full Name
TEK tyrosine kinase, endothelial
References
  • Jones N, et al. (1998) The Tek / Tie2 receptor signals through a novel Dok-related docking protein, Dok-R. Oncogene. 17(9): 1097-108.
  • Sato A, et al. (1998) Characterization of TEK receptor tyrosine kinase and its ligands, Angiopoietins, in human hematopoietic progenitor cells. Int Immunol. 10(8): 1217-27.
  • Huang L, et al. (1995) GRB2 and SH-PTP2: potentially important endothelial signaling molecules downstream of the TEK / TIE2 receptor tyrosine kinase. Oncogene. 11(10): 2097-103.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-TIE2 Antibody Rabbit Polyclonal ELISA 51087-T16
    Anti-TIE2 Antibody (APC) Mouse Monoclonal FCM 10700-MM07-A
    Anti-TIE2 Antibody Rabbit Polyclonal ELISA 10700-RP01
    Anti-TIE2 Antibody Mouse Monoclonal ELISA 10700-MM01
    Anti-TIE2 Antibody (APC) Rabbit Monoclonal FCM 10700-R116-A
    Anti-TIE2 Antibody (FITC) Mouse Monoclonal FCM 10700-MM07-F
    Anti-TIE2 Antibody (FITC) Rabbit Monoclonal FCM 10700-R116-F
    Anti-TIE2 Antibody Mouse Monoclonal FCM,ICC/IF,IF 10700-MM07
    Anti-TIE2 Antibody (PE) Mouse Monoclonal FCM 10700-MM07-P
    Anti-TIE2 Antibody (PE) Rabbit Monoclonal FCM 10700-R116-P
    Anti-TIE2 Antibody Rabbit Polyclonal ELISA 10700-RP02
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