Zika virus (strain Zika SPH2015) E / Envelope protein (Domain I, His & MBP Tag)

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Zika virus (strain Zika SPH2015) E / Envelope protein (Domain I, His & MBP Tag): 产品信息

纯度
> 75 % as determined by SDS-PAGE.
内毒素
Please contact us for more information.
生物活性
Testing in progress
蛋白构建
A DNA sequence encoding the Zika virus (strain Zika SPH2015) E (ALU33341.1) (Ile291-Thr399 and Glu426-Gly485 and Ser576- Gly592) was expressed with a N-terminal polyhistidine-tagged MBP tag at the N-terminus (his-MBP).
表达宿主
E. coli
种属
ZIKV
预测 N 端
Met
分子量
The recombinant Zika virus (strain Zika SPH2015) E consists of 523 amino acids and predicts a molecular mass of 57.2 kDa.
缓冲液
Lyophilized from sterile 250 mM NaCl, 50 mM Tris, pH 8.0.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Zika virus (strain Zika SPH2015) E / Envelope protein (Domain I, His & MBP Tag): 图片

ZIKV Envelope 背景信息

Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
参考文献
    1. Marina Basarab specialty registrar. et al .Zika virus. BMJ 2016; 352:i1049 doi: 10.1136/bmj.i1049
    2. Pinto Júnior VL, et al. Zika virus: a review to clinicians, Acta Med Port 2015 Nov-Dec;28(6):760-765
    3. L Zammarchi, et al.Zika virus infection in a traveler returning to Europe from Brazil, March 2015
    4. Arturo Galindo-Fraga, et al. Zika virus: A new epidemic on our doorstep. Rev Inves Clin.2015, 67: 329-32
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