Anti-ALDH1A1 Antibody

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Anti-ALDH1A1 Antibody (Mouse Monoclonal 抗体) 产品信息

产品名称
Anti-ALDH1A1 Antibody
经验证的应用
WB,ELISA,FCM,ICC/IF
交叉反应
Reacts with: Human
特异性
Human ALDH1A1
No cross-reactivity with E.coli cell lysate in ELISA
免疫原
Recombinant Human ALDH1 protein (Catalog#11388-H07E)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ALDH1 (rh ALDH1; Catalog#11388-H07E; NP_000680.2; Ser 2-Ser 501). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography .
来源
Monoclonal Mouse IgG1 Clone #5G9E6C9
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ALDH1A1 Antibody (Mouse Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:1000-1:2000
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ALDH1A1 Antibody (Mouse Monoclonal 抗体) 图片

Immunofluorescence staining of Human ALDH1A1 in MCF7 or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human ALDH1A1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.
Flow cytometric analysis of Human ALDH1A1 on A549 cells. Cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human ALDH1A1, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-ALDH1A1 mouse monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:55 kDa

Observed band size:55 kDa

Anti-ALDH1A1 Antibody: 别称

Anti-ALDC Antibody; Anti-ALDH-E1 Antibody; Anti-ALDH1 Antibody; Anti-ALDH11 Antibody; Anti-HEL-9 Antibody; Anti-HEL-S-53e Antibody; Anti-HEL12 Antibody; Anti-PUMB1 Antibody; Anti-RALDH1 Antibody

ALDH1A1 背景信息

Aldehyde dehydrogenase 1 family, member A1 (ALDH1A1), also known as Aldehyde dehydrogenase 1 (ALDH1), or Retinaldehyde Dehydrogenase 1 (RALDH1), is an enzyme that is expressed at high levels in stem cells and that has been suggested to regulate stem cell function. The retinaldehyde dehydrogenase (RALDH) subfamily of ALDHs, composed of ALDH1A1, ALDH1A2, ALDH1A3, and ALDH8A1, regulate development by catalyzing retinoic acid biosynthesis. The ALDH1A1 protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea. Increased ALDH1A1 activity has been found in the stem cell populations of leukemia and some solid tumors. In tumor specimens, increased ALDH1A1 immunopositivity was found not only in secretory type cancer epithelial cells but also in neuroendocrine tumor populations. ALDH1 has been identified as a reliable marker of breast cancer stem cells. ALDH1 expression in primary cancer is an independent prognostic factor in node-positive breast cancer patients. ALDH1A1 plays a key role in normal hematopoiesis, and as a TLX1 transcriptional target, ALDH1A1 may contribute to the ability of this homeoprotein to alter cell fate and induce tumor growth.
全称
aldehyde dehydrogenase 1 family, member A1
参考文献
  • Li T, et al. (2010). ALDH1A1 is a marker for malignant prostate stem cells and predictor of prostate cancer patients' outcome. Lab Invest. 90(2): 234-44.
  • Levi BP, et al. (2009) Aldehyde dehydrogenase 1a1 is dispensable for stem cell function in the mouse hematopoietic and nervous systems. Blood. 113(8): 1670-80.
  • Rahman FB, et al. (2006) Uncompetitive inhibition of Xenopus laevis aldehyde dehydrogenase 1A1 by divalent cations. Zoolog Sci. 23(3): 239-44.
  • Jester JV, et al. (1999). The cellular basis of corneal transparency: evidence for corneal crystallins. J Cell Sci. 112 ( Pt 5): 613-22.
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