Anti-c-Kit Antibody (PE)

1/1
Price:
Size:
Number:

Anti-c-Kit Antibody (PE) (Rabbit Monoclonal 抗体) 产品信息

产品名称
Anti-c-Kit Antibody (PE)
经验证的应用
FCM
交叉反应
Reacts with: Human
特异性
Human c-Kit
免疫原
Recombinant Human c-Kit / CD117 protein (Catalog#11996-H08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human c-Kit / CD117 (rh c-Kit / CD117; Catalog#11996-H08H; P10721-2; Met1-Thr516) and conjugated with PE under optimum conditions, the unreacted PE was removed.
来源
Monoclonal Rabbit IgG Clone #007
纯化
Protein A
缓冲液
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
偶联物
PE
浓度
10 μl/Test, 0.1 mg/ml
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-c-Kit Antibody (PE) (Rabbit Monoclonal 抗体) 图片

Flow cytometric analysis of human KIT (CD117) expression on TF-1 cells. TF-1 cells were stained with PE-conjugated anti-Human KIT (CD117). The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-c-Kit Antibody (PE): 别称

Anti-C-Kit Antibody; Anti-CD117 Antibody; Anti-PBT Antibody; Anti-SCFR Antibody

c-Kit 背景信息

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.
全称
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
参考文献
  • Andre C, et al. (1997) Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics. 39(2):216-26.
  • Yarden Y, et al. (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6(11):3341-51.
  • Leong KG, et al. (2008) Generation of a prostate from a single adult stem cell. Nature. 456(7223): 804-8.
  • Edling CE, et al. (2007) c-Kit--a hematopoietic cell essential receptor tyrosine kinase. Int J Biochem Cell Biol. 39(11):1995-8.
  • McIntyre A, et al. (2005) Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults. Cancer Res. 65(18):8085-9.
  • Establishment and Characterization of a Highly Metastatic Ovarian Cancer Cell Line
    Author
    Ruibin, J;Guoping, C;Zhiguo, Z;Maowei, N;Danying, W;Jianguo, F;Linhui, G;
    Year
    2018
    Journal
    Biomed Res Int
    Application
    FAC
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后