IL17 Protein, Canine, Recombinant

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IL17 Protein, Canine, Recombinant: 产品信息

纯度
> 95 % as determined by SDS-PAGE
内毒素
Please contact us for more information.
生物活性
1. Measured by its binding ability in a functional ELISA. Immobilized Canine IL17A (cat: 70102-DNAE) at 10 μg/ml (100 μl/well) can bind human IL17Ra (Cat:10895-H03H), The EC50 of human IL17Ra (Cat:10895-H03H) is 6.0-15.0 ng/ml.
2. Measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells. The ED50 for this effect is typically 1-5 ng/mL.
蛋白构建
A DNA sequence encoding the canine IL17A (NP_001159350.1)(Gly26-Ala155) was expressed with a N-terminal Met.
表达宿主
E. coli
种属
Canine
预测 N 端
Met
分子量
The recombinant canine IL17A comprises 131 amino acids and has a predicted molecular mass of 15.2 kDa. The apparent molecular mass of the protein is approximately 15 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL17 Protein, Canine, Recombinant: 图片

IL17 背景信息

IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
全称
interleukin 17A
参考文献
  • Andoh A, et al. (2002) IL-17 selectively down-regulates TNF-alpha-induced RANTES gene expression in human colonic subepithelial myofibroblasts. J Immunol. 169(4):1683-7.
  • Kotake S, et al. (1999) IL-17 in synovial fluids from patients with rheumatoid arthritis is a potent stimulator of osteoclastogenesis. J Clin Invest. 103(9):1345-52.
  • Laan M, et al. (1999) Neutrophil recruitment by human IL-17 via C-X-C chemokine release in the airways. J Immunol. 162(4):2347-52.
  • Shin HC, et al. (1999) Regulation of IL-17, IFN-gamma and IL-10 in human CD8(+) T cells by cyclic AMP-dependent signal transduction pathway. Cytokine. 10(11):841-50.
  • The effects of interleukin 17A on left stellate ganglion remodeling are mediated by neuroimmune communication in normal structural hearts
    Author
    Deng, J;Zhou, X;Wang, M;Wang, M;Zhou, L;Meng, G;Wang, Y;Wang, Z;Zhang, Y;Zhou, Z;Yu, L;Jiang, H;
    Year
    2019
    Journal
    Int. J. Cardiol.
    Application
    animal-based
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