IL3RA Protein, Human, Recombinant (His Tag)

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IL3RA Protein, Human, Recombinant (His Tag): 产品信息

纯度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its binding ability in a functional ELISA . Immobilized human IL3 at 20 μg/ml (100 μl/well) can bind biotinylated human IL3RA with a linear ranger of 0.32-1.6 μg/ml.
蛋白构建
A DNA sequence encoding the human IL3RA (NP_002174.1) extracellular domain (Met 1-Arg 305) was expressed, with a C-terminal polyhistidine tag.
NP No.
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Thr 19
分子量
The secreted recombinant human IL3RA comprises 298 amino acids and has a predicted molecular mass of 34.5 kDa. As a result of glycosylation, the apparent molecular mass of rh IL3RA is approximately 50-60 kDa in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL3RA Protein, Human, Recombinant (His Tag): 图片

IL3RA Protein, Human, Recombinant (His Tag): 别称

CD123 Protein, Human; hIL-3Ra Protein, Human; IL3R Protein, Human; IL3RAY Protein, Human; IL3RX Protein, Human; IL3RY Protein, Human

IL3RA 背景信息

Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and / or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.
全称
interleukin 3 receptor, alpha (low affinity)
参考文献
  • Del Giudice I, et al. (2004) The diagnostic value of CD123 in B-cell disorders with hairy or villous lymphocytes. Haematologica. 89(3): 303-8.
  • Du X, et al. (2007) New immunotoxins targeting CD123, a stem cell antigen on acute myeloid leukemia cells. J Immunother. 30(6): 607-13.
  • Yue LZ, et al. (2010) Expression of CD123 and CD114 on the bone marrow cells of patients with myelodysplastic syndrome. Chin Med J (Engl). 123(15): 2034-2037.
  • Optimization of human NK cell manufacturing: Fully-automated separation, improved <i>ex vivo </i>expansion using IL-21 with autologous feeder cells and generation of anti-CD123-CAR-expressing effector cells
    Author
    Klöß, S;Oberschmidt, O;Morgan, M;Dahlke, J;Arseniev, L;Huppert, V;Granzin, M;Gardlowski, T;Matthies, N;Soltenborn, S;Schambach, A;Koehl, U;
    Year
    2017
    Journal
    Hum. Gene Ther.
    Application
    CAR-T cell detection
  • Programmed cell death protein 1 activation preferentially inhibits CD28.CAR-T cells
    Author
    Zolov, SN;Rietberg, SP;Bonifant, CL;
    Year
    2018
    Journal
    Cytotherapy
    Application
    FCM (CAR-T)
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