Anti-CD64 Antibody

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Anti-CD64 Antibody (Rabbit Monoclonal 抗体) 产品信息

产品名称
Anti-CD64 Antibody
经验证的应用
ELISA,IHC-P
交叉反应
Reacts with: Mouse
特异性
Mouse CD64
Has cross-reactivity in ELISA with
Human CD64 / FCGR1A
No cross-reactivity in ELISA with
Mouse CD16-2 /FCGR4
Mouse FCGR2B / CD32
Mouse FCGR3 / CD16
Human CD16a / FCGR3A
Human CD16b / FCGR3B
Human CD32a / FCGR2A
Human CD32b / FCGR2B
免疫原
Recombinant Mouse FCGR1 protein (Catalog#50086-M08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FCGR1 extracellular domain (rM FCGR1; Catalog#50086-M08H; NP_034316.1; Met 1-Pro 297).
来源
Monoclonal Rabbit IgG Clone #1
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD64 Antibody (Rabbit Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
ELISA 1:5000-1:10000
IHC-P 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD64 Antibody (Rabbit Monoclonal 抗体) 图片

Immunochemical staining of mouse FCGR1 in mouse spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Immunochemical staining of mouse FCGR1 in mouse liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to Kupffer cell.
Immunochemical staining of mouse FCGR1 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to microglia.

Anti-CD64 Antibody: 别称

Anti-AI323638 Antibody; Anti-AV092959 Antibody; Anti-CD64 Antibody; Anti-FcgammaRI Antibody; Anti-IGGHAFC Antibody

CD64 背景信息

High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.
全称
Fc receptor, IgG, high affinity I
参考文献
  • Allen J.M., et al.,(1988), Nucleotide sequence of three cDNAs for the human high affinity Fc receptor (FcRI). Nucleic Acids Res. 16:11824-11824.
  • Allen J.M., et al., (1989), Isolation and expression of functional high-affinity Fc receptor complementary DNAs.Science 243:378-381.
  • Porges A.J., et al.,(1992), Novel Fc gamma receptor I family gene products in human mononuclear cells.J. Clin. Invest. 90:2102-2109.
  • Cartilage-binding antibodies induce pain through immune complex-mediated activation of neurons
    Author
    Bersellini Farinotti, A;Wigerblad, G;Nascimento, D;Bas, DB;Morado Urbina, C;Nandakumar, KS;Sandor, K;Xu, B;Abdelmoaty, S;Hunt, MA;Ängeby Möller, K;Baharpoor, A;Sinclair, J;Jardemark, K;Lanner, JT;Khmaladze, I;Borm, LE;Zhang, L;Wermeling, F;Cragg, MS;Lengqvist, J;Chabot-Doré, AJ;Diatchenko, L;Belfer, I;Collin, M;Kultima, K;Heyman, B;Jimenez-Andrade, JM;Codeluppi, S;Holmdahl, R;Svensson, CI;
    Year
    2019
    Journal
    J. Exp. Med.
    Application
    IHC
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