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eIF5A Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag

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eIF5A Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag: 产品信息

基因
种属
Mouse
NCBI 参考序列号
基因长度
465 bp
序列特征
Identical with the Gene Bank Ref. ID sequence (Nucleotide may contain silent mutation without changing amino acid sequence)
产品特征
Full length Clone DNA of Mus musculus eukaryotic translation initiation factor 5A.
质粒
启动子
Enhanced CMV mammalian cell promoter
标签序列
GFPSpark Tag Sequence: GTGAGCAAGGGC……GAGCTGTACAAG
测序引物
pLen-F(CTCGTTTAGTGAACCGTCAGAATT), pLen-R(GAACCGGAACCCTTAAACATGT)
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Ampicillin
储存 & 运输
运输方式
Each tube contains 10μg lyophilized plasmid
储存条件
The lyophilized plasmid can be stored at room temperature for three months

eIF5A Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag: 别称

AA410058 cDNA ORF Clone, Mouse; D19Wsu54e cDNA ORF Clone, Mouse; eIF-4D cDNA ORF Clone, Mouse; eIF-5A cDNA ORF Clone, Mouse; eIF-5A-1 cDNA ORF Clone, Mouse; eIF-5A1 cDNA ORF Clone, Mouse; Eif4d cDNA ORF Clone, Mouse; Eif5a1 cDNA ORF Clone, Mouse

eIF5A 背景信息

EIF-5A, also known as EIF5, functions in start site selection as a GTPase accelerating protein (GAP) for the eukaryotic translation initiation factor (eIF) 2•GTP•tRNA ternary complex within the ribosome-bound pre-initiation complex. In protein synthesis initiation, eIF2 functions in its GTP-bound state to deliver initiator methionyl-tRNA to the small ribosomal subunit and is necessary for protein synthesis in all cells. EIF-5A stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). EIF-5A also interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 4S ribosome subunit.
全称
eukaryotic translation initiation factor 5A
参考文献
  • Jenkins ZA. et al., 2001, Genomics. 71 (1): 101-9.
  • Guan XY. et al., 2001, Cancer Res. 61 (9): 3806-9.
  • Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903.
  • Clement PM. et al., 2003, Eur J Biochem. 270 (21): 4254-63.
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