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SHP2 cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag

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SHP2 cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: 产品信息

基因
种属
Mouse
NCBI 参考序列号
基因长度
1782 bp
序列特征
Identical with the Gene Bank Ref. ID sequence.
产品特征
Full length Clone DNA of Mouse protein tyrosine phosphatase, non-receptor type 11, transcript variant 2 with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + XbaI (6kb + 1.82kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

SHP2 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

SHP2 cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: 验证图片

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

SHP2 cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: 别称

2700084A17Rik cDNA ORF Clone, Mouse; AW536184 cDNA ORF Clone, Mouse; PTP1D cDNA ORF Clone, Mouse; PTP2C cDNA ORF Clone, Mouse; SAP-2 cDNA ORF Clone, Mouse; SH-PTP2 cDNA ORF Clone, Mouse; SH-PTP3 cDNA ORF Clone, Mouse; SHP-2 cDNA ORF Clone, Mouse; Shp2 cDNA ORF Clone, Mouse; Syp cDNA ORF Clone, Mouse

SHP2 背景信息

SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase(PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.
全称
protein tyrosine phosphatase, non-receptor type 11
参考文献
  • Ganju R K, et al. (2000) Beta-chemokine receptor CCR5 signals through SHP1, SHP2, and Syk. J Biol Chem. 275(23):17263-8.
  • Yin T, et al. (1997) Molecular characterization of specific interactions between SHP-2 phosphatase and JAK tyrosine kinases. J Biol Chem. 272(2):1032-7.
  • Kontaridis MI, et al. (2006) PTPN11 (Shp2) mutations in LEOPARD syndrome have dominant negative, not activating, effects. J Biol Chem. 281(10):6785-92.
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