Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit MAb

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Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit MAb (Rabbit Monoclonal 抗体) 产品信息

产品名称
Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit MAb
经验证的应用
WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
应用说明
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
特异性
CMV CMV Glycoprotein B
免疫原
Recombinant Human CMV gB / Glycoprotein B protein (Catalog#10202-V08H1)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human CMV gB (Catalog#10202-V08H1; AAA45920.1) extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907).
来源
Monoclonal Rabbit IgG Clone #38
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit MAb (Rabbit Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect Human CMV gB in WB. Using a DAB detection system, the detection limit for Human CMV gB is approximately 0.125 ng/lane under non-reducing conditions and 100 ng/lane under reducing conditions.
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

CMV Glycoprotein B 背景信息

Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.
参考文献
  • Lopper M,et al. (2002). Disulfide bond configuration of human cytomegalovirus glycoprotein B. J Virol. 76(12): 6073-82.
  • Isaacson MK, et al. (2009) Human cytomegalovirus glycoprotein B is required for virus entry and cell-to-cell spread but not for virion attachment, assembly, or egress. J Virol. 83(8): 3891-903.
  • Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells
    Author
    Smargiasso, N;Nader, J;Rioux, S;Mazzucchelli, G;Boutry, M;De Pauw, E;Chaumont, F;Navarre, C;
    Year
    2019
    Journal
    Int J Mol Sci
    Application
    WB
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