Anti-EGFR Antibody

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Anti-EGFR Antibody (Rabbit Monoclonal 抗体) 产品信息

产品名称
Anti-EGFR Antibody
经验证的应用
WB,ELISA,ELISA(Det),ICC/IF,IF
交叉反应
Reacts with: Human
特异性
Human EGFR
No cross-reactivity in ELISA with
Human ErbB2 / HER2
Human ErbB3 / HER3
免疫原
Recombinant Human EGFR Protein(Catalog#10001-H08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR (rh EGFR; Catalog#10001-H08H; Met 1-Ser 645; NP_005219) .
来源
Monoclonal Rabbit IgG Clone #021
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-EGFR Antibody (Rabbit Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
ICC-IF 1:100-1:500
ELISA(Det) 1:5000-1:50000
Notes
ELISA(Det): This antibody will detect Human EGFR / HER1 in ELISA pair set (Catalog: # SEK10001). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10001-RE11).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-EGFR Antibody (Rabbit Monoclonal 抗体) 图片

Anti-EGFR rabbit monoclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:180 kDa

Observed band size:180 kDa

Immunofluorescence staining of Human EGFR in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human EGFR monoclonal antibody (1:300) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

Anti-EGFR Antibody: 别称

Anti-ERBB Antibody; Anti-ERBB1 Antibody; Anti-HER1 Antibody; Anti-mENA Antibody; Anti-NISBD2 Antibody; Anti-PIG61 Antibody

EGFR 背景信息

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
全称
epidermal growth factor receptor
参考文献
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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