EGFR Protein, Human, Recombinant (His Tag)

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EGFR Protein, Human, Recombinant (His Tag): 产品信息

纯度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its binding ability in a functional ELISA.
Immobilized recombinant human EGFR (Cat:10001-H08H) at 10 μg/ml(100 μl/well) can bind human Fc-EGF (Cat:10605-H01H) with a linear range of 1.28-20 μg/ml.
蛋白构建
A DNA sequence encoding the extracellular domain (Met 1-Ser 645) of human EGFR (NP_005219) was expressed with a C-terminal polyhistidine tag.
NP No.
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Leu 25
分子量
The recombinant human EGF receptor consists of 630 amino acids and has a calculated molecular mass of 69.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110 kDa protein in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EGFR Protein, Human, Recombinant (His Tag): 图片

Measured by its binding ability in a functional ELISA. Immobilized recombinant human EGFR (Cat:10001-H08H) at 10 μg/ml(100 μl/well) can bind human Fc-EGF (Cat:10605-H01H) with a linear range of 1.28-20 μg/ml.

EGFR Protein, Human, Recombinant (His Tag): 别称

ERBB Protein, Human; ERBB1 Protein, Human; HER1 Protein, Human; mENA Protein, Human; NISBD2 Protein, Human; PIG61 Protein, Human

EGFR 背景信息

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
全称
epidermal growth factor receptor
参考文献
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
  • Phage-derived fully human antibody scFv fragment directed against human vascular endothelial growth factor receptor 2 blocked its interaction with VEGF
    Author
    Zhang, J;Li, H;Wang, X;Qi, H;Miao, X;Zhang, T;Chen, G;Wang, M;
    Year
    2012
    Journal
    Biotechnol. Prog.
    Application
    binding (negative control)
  • Phage‐derived fully human antibody scFv fragment directed against human vascular endothelial growth factor receptor 2 blocked its interaction with VEGF
    Author
    J Zhang, H Li, X Wang, H Qi, X Miao…
    Year
    2012
    Journal
    Biotechnol. Prog
    Application
    negative control in the test of the specificity of scFv antibodies
  • Erbin interacts with c-Cbl and promotes tumourigenesis and tumour growth in colorectal cancer by preventing c-Cbl-mediated ubiquitination and down-regulation of EGFR
    Author
    Yao, S;Zheng, P;Wu, H;Song, LM;Ying, XF;Xing, C;Li, Y;Xiao, ZQ;Zhou, XN;Shen, T;Chen, L;Liu, YH;Lai, M;Mei, L;Gao, TM;Li, JM;
    Year
    2014
    Journal
    J. Pathol.
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