< 1.0 EU per μg of the protein as determined by the LAL method
Measured by its binding ability in a functional ELISA. Immobilized recombinant human EGFR (Cat:10001-H08H） at 10 μg/ml(100 μl/well) can bind human Fc-EGF (Cat:10605-H01H) with a linear range of 1.28-20 μg/ml.
A DNA sequence encoding the extracellular domain (Met 1-Ser 645) of human EGFR (NP_005219) was expressed with a C-terminal polyhistidine tag.
The recombinant human EGF receptor consists of 630 amino acids and has a calculated molecular mass of 69.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110 kDa protein in SDS-PAGE under reducing conditions.
Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EGFR Protein, Human, Recombinant (His Tag): 图片
Measured by its binding ability in a functional ELISA.
Immobilized recombinant human EGFR (Cat:10001-H08H） at 10 μg/ml(100 μl/well) can bind human Fc-EGF (Cat:10605-H01H) with a linear range of 1.28-20 μg/ml.
EGFR Protein, Human, Recombinant (His Tag): 别称
ERBB Protein, Human; ERBB1 Protein, Human; HER1 Protein, Human; mENA Protein, Human; NISBD2 Protein, Human; PIG61 Protein, Human
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.