Anti-EpCAM rabbit polyclonal antibody at 1:500 dilution
Lane A: A431 Whole Cell Lysate
Lane B: Caco2 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:35 kDa
Observed band size:35 kDa
(We are unsure as to the identity of these extra bands.)
EpCAM was immunoprecipitated using:
Lane A:0.5 mg MCF-7 Whole Cell Lysate
Lane B:0.5 mg A431 Whole Cell Lysate
Lane C:0.5 mg Caco-2 Whole Cell Lysate0.5 µL anti-EpCAM rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-EpCAM rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 40 kDa
Observed band size: 40 kDa