Human Immunodeficiency Virus type 1 (HIV-1) gp120 Antibody, Mouse MAb

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Human Immunodeficiency Virus type 1 (HIV-1) gp120 Antibody, Mouse MAb (Mouse Monoclonal 抗体) 产品信息

产品名称
Human Immunodeficiency Virus type 1 (HIV-1) gp120 Antibody, Mouse MAb
经验证的应用
WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
应用说明
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
特异性
HIV HIV gp120
免疫原
Recombinant HIV-1 gp120 Protein (Catalog#11233-V08H)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant HIV-1 gp120 (Catalog#11233-V08H; Ala 29-Glu 503; 97CN001 strain). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG2b Clone #4F4E4
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Human Immunodeficiency Virus type 1 (HIV-1) gp120 Antibody, Mouse MAb (Mouse Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect HIV-GP120-His in WB.
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

HIV gp120 背景信息

The HIV-1 gp120 envelope protein, a glycoprotein that is part of the outer layer of the virus, which is an essential component in the multi-tiered viral entry process. It presents itself as viral membrane spikes consisting of 3 molecules of gp120 linked together and anchored to the membrane by gp41 protein. Gp120 is essential for viral infection as it facilitates HIV entry into the host cell and this is its best-known and most researched role in HIV infection. However, it is becoming increasingly evident that gp12 might also be facilitating viral persistence and continuing HIV infection by influencing the T cell immune response to the virus. The surface protein gp120 attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. Gp120 binding to its receptor CD4 and co-receptor, CXCR4 or CCR5 is required for fusion of viral and cellular membranes. Several mechanisms might be involved in this process of which gp120 binding to the CD4 receptor of T cells is the best known and most important interaction as it facilitates viral entry into the CD4+ cells and their depletion, a hallmark of the HIV infection. Gp120 is shed from the viral membrane and accumulates in lymphoid tissues in significant amounts. Despite the overall genetic heterogeneity of the gp120 glycoprotein, the conserved CD4 binding site provides an attractive antiviral target. Interaction between gp120 and ITGA4/ITGB7 would allow the virus to enter GALT early in the infection, infecting and killing most of GALT's resting CD4+ T-cells. This T-cell depletion is believed to be the major insult to the host immune system leading to AIDS.
参考文献
  • Kadow J, et al. (2006) Small-molecule HIV-1 gp120 inhibitors to prevent HIV-1 entry: an emerging opportunity for drug development. Curr Opin Investig Drugs. 7(8): 721-6.
  • Stevceva L, et al. (2007) Immune responses to HIV Gp120 that facilitate viral escape. Curr HIV Res. 5(1): 47-54.
  • Yoon V, et al. (2010) The GP120 molecule of HIV-1 and its interaction with T cells. Curr Med Chem. 17(8): 741-9.
  • Enabling electrical biomolecular detection in high ionic concentrations and enhancement of the detection limit thereof by coupling a nanofluidic crystal with reconfigurable ion concentration polarization
    Author
    Ouyang, W;Han, J;Wang, W;
    Year
    2017
    Journal
    Lab Chip
    Application
    NFC-ICP; capture antigen
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