L1CAM cDNA ORF Clone, Human, C-HA tag

1/1
Price:
Size:
Number:

L1CAM cDNA ORF Clone, Human, C-HA tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
3762 bp
序列特征
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1977 T>C not causing the amino acid variation.
产品特征
Full length Clone DNA of Human L1 cell adhesion molecule (L1CAM), transcript variant 2 with C terminal HA tag.
质粒
启动子
Enhanced CMV promoter
载体
限制性酶切位点
HindIII + XbaI (6kb + 3.81kb)
标签序列
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

L1CAM cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

L1CAM cDNA ORF Clone, Human, C-HA tag: 验证图片

L1CAM cDNA ORF Clone, Human, C-HA tag: 别称

CAML1 cDNA ORF Clone, Human; CD171 cDNA ORF Clone, Human; HSAS cDNA ORF Clone, Human; HSAS1 cDNA ORF Clone, Human; MASA cDNA ORF Clone, Human; MIC5 cDNA ORF Clone, Human; N-CAM-L1 cDNA ORF Clone, Human; N-CAML1 cDNA ORF Clone, Human; NCAM-L1 cDNA ORF Clone, Human; S10 cDNA ORF Clone, Human; SPG1 cDNA ORF Clone, Human

L1CAM 背景信息

L1 cell adhesion molecule (L1CAM), also designated as CD171, is a cell adhesion receptor of the immunoglobulin superfamily, known for its roles in nerve cell function. While originally believed to be present only in brain cells, in recent years L1-CAM has been detected in other tissues, and in a variety of cancer cells, including some common types of human cancer. L1CAM interacts with a variety of ligands including axonin-1, CD9, neurocan and intergrins, and it has been revealed that the RGD motif in the sixth Ig domain of L1CAM is a binding site for integrins, thus important for nuclear signaling. Disruption of L1CAM function causes three X-linked neurological syndromes, i.e. hydrocephalus, MASA syndrome (mental retardation, aphasia, shuffling gait and adducted thumbs) and spastic paraplegia syndrome. Overexpression of L1CAM in normal and cancer cells increased motility, enhanced growth rate and promoted cell transformation and tumorigenicity. Recent work has identified L1CAM (CD171) as a novel marker for human carcinoma progression, and a candidate for anti-cancer therapy.
全称
L1 cell adhesion molecule
参考文献
  • Meier F, et al. (2006) The adhesion molecule L1 (CD171) promotes melanoma progression. Int J Cancer. 119(3): 549-55.
  • Gavert N, et al. (2008) L1-CAM in cancerous tissues. Expert Opin Biol Ther. 8(11): 1749-57.
  • Issa Y, et al. (2009) Enhanced L1CAM expression on pancreatic tumor endothelium mediates selective tumor cell transmigration. J Mol Med. 87(1): 99-112.
  • Weidle UH, et al. (2009) L1-CAM as a target for treatment of cancer with monoclonal antibodies. Anticancer Res. 29(12): 4919-31.
  • Raveh S, et al. (2009) L1 cell adhesion molecule (L1CAM) in invasive tumors. Cancer Lett. 282(2): 137-45.
  • Wolterink S, et al. (2010) Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer Res. 70(6): 2504-15.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后