PADI4 cDNA ORF Clone, Human, C-Myc tag

Cat: HG11072-M-M
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PADI4 cDNA ORF Clone, Human, C-Myc tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
1992 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for two point mutations 245 T/C and 349 T/C not causing the amino acid variation.
描述
Full length Clone DNA of Human peptidyl arginine deiminase, type I V with Myc tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV2-Myc
限制性酶切位点
KpnI + XbaI (5.4kb + 2.04kb)
标签序列
Myc Tag Sequence: CAGAAACTCATCTCAGAAGAGGATCTG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
PADI4 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

PADI4 cDNA ORF Clone, Human, C-Myc tag Validated Images
Human Integrin alpha 5/CD49e/ITGA5 Gene Plasmid Map 7251
PADI4 cDNA ORF Clone, Human, C-Myc tag Alternative Names
PAD cDNA ORF Clone, Human;PAD4 cDNA ORF Clone, Human;PADI5 cDNA ORF Clone, Human;PDI4 cDNA ORF Clone, Human;PDI5 cDNA ORF Clone, Human
PADI4 Background Information

Protein-arginine deiminase type-4, also known as HL-60 PAD, Peptidylarginine deiminase IV, Protein-arginine deiminase type I V and PADI4, is a cytoplasm and nucleus protein which belongs to the protein arginine deiminase family. PADI4 is expressed in CD34+ stem cells in normal tissues, and many more CD34+ cells expressing PADI4 are present in tumour tissues. PADI4 post-translationally converts peptidylarginine to citrulline, a process called citrullination. Studies have demonstrated the high expression of PADI4 in various malignant tumour tissues. PADI4 is also expressed at high levels in the blood of patients with some malignant tumours. Citrullination of histone, cytokeratin, antithrombin and fibronectin have been confirmed to be involved in abnormal apoptosis, high coagulation, and disordered cell proliferation and differentiation, all of which are main features of malignant tumours. PADI4 may play an important role in tumourigenesis. Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.

Full Name
peptidyl arginine deiminase, type IV
References
  • Nakashima K. et al.,1999, J. Biol. Chem. 274: 27786-92.
  • Suzuki A. et al., 2003, Nat. Genet. 34:395-402.
  • Nakayama-Hamada M. et al., 2005, Biochem. Biophys. Res. Commun. 327:192-200.
  • Chang, X. et al., 2010, Cancer Cell Int  10:7.
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