Von Willebrand Factor / vWF ELISA Kit, Human

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Von Willebrand Factor / vWF ELISA Kit, Human: 产品信息

产品名称
Von Willebrand Factor / vWF ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
155.29 pg/mL
线性范围
312.5-20000 pg/mL
特异性
Recognizes both recombinant and natural Human Von Willebrand Factor / vWF
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 2,074.19 4,023.55 8,366.13
SD 123.01 248.21 502.56
CV(%) 5.9% 6.2% 6.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 2,216.70 3,880.11 8,496.22
SD 199.73 306.14 340.03
CV(%) 9.0% 7.9% 4.0%
回收率
The recovery of Human vWF spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
108
Range
107 -109%
线性关系
Serum
  Recovery of detected
1:2 90%
1:4 100%
1:8 100%
1:16 104%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Von Willebrand Factor / vWF ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Von Willebrand Factor / vWF protein in Serum . It contains recombinant Human Von Willebrand Factor / vWF, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Von Willebrand Factor / vWF ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Von Willebrand Factor / vWF ELISA Kit, Human: 别称

F8VWF ELISA Kit, Human; Von Willebrand Factor ELISA Kit, Human; VWD ELISA Kit, Human

Von Willebrand Factor / vWF 背景信息

Von Willebrand Factor (VWF) is a multimeric glycoprotein involved in hemostasis in blood, binds receptors on the surface of platelets and in connective tissue, thereby mediating the adhesion of platelets to sites of vascular injury. From studies it appears that VWF protein uncoils under these circumstances, decelerating passing platelets. VWF protein is deficient or defective in von Willebrand disease (VWD) and is involved in a large number of other diseases, including thrombosis, thrombotic thrombocytopenic purpura, Stroke, Heyde's syndrome, possibly hemolytic-uremic syndrome and so on.
全称
von Willebrand factor
参考文献
  • Sadler JE. (1998) Biochemistry and genetics of von Willebrand factor. Annu Rev Biochem, 67: 395-424.
  • Batlle J, et al. (2009) Von Willebrand factor/factor VIII concentrates in the treatment of von Willebrand disease. Blood Coagul Fibrinolysis. 20(2):89-100.
  • Sadler JE. (2009) von Willebrand factor assembly and secretion. J Thromb Haemost. 7 Suppl 1:24-7.
  • Auton M, et al. (2010) The mechanism of VWF-mediated platelet GPIbalpha binding. Biophys J. 99(4):1192-201.
  • Bowen DJ. (2010) Sugar targets VWF for the chop. Blood. 115(13):2565.
  • Lpez JA, et al. (2010) VWF self-association: more bands for the buck. Blood. 116(19):3693-4.
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