Anti-gp130 / IL6ST Antibody

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Anti-gp130 / IL6ST Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-gp130 / IL6ST Antibody
经验证的应用
IHC-P,ICC/IF
交叉反应
Reacts with: Rhesus
特异性
Rhesus gp130 / IL6ST
免疫原
Recombinant Rhesus IL6ST / gp130 / CD130 Protein (Catalog#90275-C08H)
制备方法
Produced in rabbits immunized with purified, recombinant Rhesus IL6ST / gp130 / CD130 ( Catalog#90275-C08H; NP_001252920.1; Met1-Ile681). IL6ST / gp130 / CD130 specific IgG was purified by Rhesus IL6ST / gp130 / CD130 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-gp130 / IL6ST Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
IHC-P 1:500-1:2000
ICC-IF 1:300-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-gp130 / IL6ST Antibody (Rabbit Polyclonal 抗体) 图片

Immunofluorescence staining of IL6ST in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Rhesus IL6ST polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to cytoplasm and nucleus.
Immunochemical staining of Rhesus IL6ST in Rhesus kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining of Rhesus IL6ST in Rhesus brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

gp130 / IL6ST 背景信息

Glycoprotein 13 (also known as gp13, IL6ST, IL6-beta or CD13) is a transmembrane protein which is the founding member of the class of all cytokine receptors. CD13/gp13 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD13/gp13 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD13/gp13 plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp13 for initiating signal transmission. CD13/gp13 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD13/gp13 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.
全称
interleukin 6 signal transducer
参考文献
  • Hibi, et al. (1990) Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell. 63 (6): 1149-57.
  • Kim H, et al. (1997) Transmembrane domain of gp130 contributes to intracellular signal transduction in hepatic cells. J Biol Chem. 272 (49): 30741-7.
  • Giordano V, et al. (1997) Shc mediates IL-6 signaling by interacting with gp130 and Jak2 kinase. J Immunol. 158 (9): 4097-103.
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