Anti-KRAS Antibody

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Anti-KRAS Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-KRAS Antibody
经验证的应用
WB
交叉反应
Reacts with: Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
特异性
Human KRAS
免疫原
A synthetic peptide corresponding to the center region of the Human K-Ras
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human K-Ras, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-KRAS Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-KRAS Antibody (Rabbit Polyclonal 抗体) 图片

Anti-KRAS rabbit polyclonal antibody at 1:500 dilution.

Lane A: Mouse lung tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:22 kDa

Observed band size:22 kDa

(We are unsure as to the identity of these extra bands.)

Anti-KRAS Antibody: 别称

Anti-C-K-RAS Antibody; Anti-CFC2 Antibody; Anti-K-RAS Antibody; Anti-K-RAS2A Antibody; Anti-K-RAS2B Antibody; Anti-K-RAS4A Antibody; Anti-K-RAS4B Antibody; Anti-KI-RAS Antibody; Anti-KRAS1 Antibody; Anti-KRAS2 Antibody; Anti-NS Antibody; Anti-NS3 Antibody; Anti-RALD Antibody; Anti-RASK2 Antibody

KRAS 背景信息

K-Ras belongs to the small GTPase superfamily, Ras family. As other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. It binds to GTP in the active state and possesses an intrinsic enzymatic activity which cleaves the terminal phosphate of the nucleotide converting it to GDP. Upon conversion of GTP to GDP, K-Ras is turned off. The rate of conversion is usually slow but can be sped up dramatically by an accessory protein of the GTPase activating protein class, for example RasGAP. In turn K-Ras can bind to proteins of the Guanine Nucleotide Exchange Factor class, for example SOS1, which forces the release of bound nucleotide. Subsequently, K-Ras binds GTP present in the cytosol and the GEF is released from ras-GTP. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations have been found to be associated with Noonan syndrome[4] and cardio-facio-cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer and lung cancer.
全称
Kirsten rat sarcoma viral oncogene homolog
参考文献
  • Ling J, et al. (2012) KrasG12D-induced IKK2//NF-B activation by IL-1 alpha and p62 feedforward loops is required for development of pancreatic ductal adenocarcinoma. Cancer Cell. 21(1):105-20.
  • Matallanas D, et al. (2011) Mutant K-Ras activation of the proapoptotic MST2 pathway is antagonized by wild-type K-Ras. Mol Cell. 44(6):893-906.
  • Regala RP, et al. (2011) Matrix metalloproteinase-10 promotes Kras-mediated bronchio-alveolar stem cell expansion and lung cancer formation. PLoS One. 6(10):e26439.
  • EPHA2 feedback activation limits the response to PDEδ inhibition in KRAS-dependent cancer cells
    Author
    Chen, YH;Lv, H;Shen, N;Wang, XM;Tang, S;Xiong, B;Ding, J;Geng, MY;Huang, M;
    Year
    2019
    Journal
    Acta Pharmacol. Sin.
    Application
    WB
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