Anti-LEP mouse monoclonal antibody at 1:500 dilution
Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 μg per lane.
Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:16 kDa
Observed band size:14 kDa
(We are unsure as to the identity of these extra bands.)