eIF5A cDNA ORF Clone, Mouse, C-Myc tag

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eIF5A cDNA ORF Clone, Mouse, C-Myc tag: 产品信息

基因
种属
Mouse
NCBI 参考序列号
基因长度
465 bp
产品特征
Full length Clone DNA of Mouse eukaryotic translation initiation factor 5A with C terminal Myc tag.
质粒
启动子
Enhanced CMV promoter
载体
标签序列
Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

eIF5A cDNA ORF Clone, Mouse, C-Myc tag: 别称

AA410058 cDNA ORF Clone, Mouse; D19Wsu54e cDNA ORF Clone, Mouse; eIF-4D cDNA ORF Clone, Mouse; eIF-5A cDNA ORF Clone, Mouse; eIF-5A-1 cDNA ORF Clone, Mouse; eIF-5A1 cDNA ORF Clone, Mouse; Eif4d cDNA ORF Clone, Mouse; Eif5a1 cDNA ORF Clone, Mouse

eIF5A 背景信息

EIF-5A, also known as EIF5, functions in start site selection as a GTPase accelerating protein (GAP) for the eukaryotic translation initiation factor (eIF) 2•GTP•tRNA ternary complex within the ribosome-bound pre-initiation complex. In protein synthesis initiation, eIF2 functions in its GTP-bound state to deliver initiator methionyl-tRNA to the small ribosomal subunit and is necessary for protein synthesis in all cells. EIF-5A stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). EIF-5A also interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 4S ribosome subunit.
全称
eukaryotic translation initiation factor 5A
参考文献
  • Jenkins ZA. et al., 2001, Genomics. 71 (1): 101-9.
  • Guan XY. et al., 2001, Cancer Res. 61 (9): 3806-9.
  • Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903.
  • Clement PM. et al., 2003, Eur J Biochem. 270 (21): 4254-63.
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