SMM 293-TI,推荐用于HEK293细胞的瞬时转染、细胞培养以及重组蛋白质的表达

产品描述:
SMM 293-TI培养基是用于HEK293细胞悬浮培养和目标分子表达的培养基。产品中含有谷氨酰胺、HEPES、碳酸氢钠、氨基酸、维生素、酚红、微量元素、PluronicÒ F-68等有利于HEK293细胞生长和表达的组分。产品无血清,无抗生素,无动物源性组分,有利于建立稳定的HEK293细胞表达系统。在转染后的表达阶段同时配套使用加料液SMS 293-SUPI能够实现细胞的长时间培养,获得更高的蛋白产量。
产品特点:
• HEK293细胞瞬时转染专用培养基 • 即用型完全培养基,不用添加其他组分
• 转染前后无需更换培养基 • 无动物源组分、无血清、无抗生素
• 适用于瞬时转染和稳定株转染以及培养 • 适用于细胞的悬浮培养和大规模的反应器培养
• 数千种蛋白及抗体成功表达案例  
产品参数:
外观 澄清红色溶液 适用细胞株 HEK293EBNA及相关的细胞(如HEK293F、HEK293E、HEK293H 、HEK293S、HEK293FT)
渗透压 275±10 mOsm/kg 细胞接种密度 0.3-0.4×106 cells/mL
pH值 6.9–7.4 倍增时间 < 26 hrs(因细胞类型、状态影响而存在数值变化)
无菌检测 无菌 细胞培养评价 优良
内毒素 < 10 EU/mL 产品用途 仅用于科学研究,不推荐用于人类或动物的诊断和治疗
有效期 6个月 储存条件 2-8 ℃,避光
SMM 293-TI培养基为澄清红色透明液体,2~8℃避光保存,使用过程中请勿反复预热。
产品使用效果:
细胞密度高
相同条件下,分别使用SMM 293-TI培养基和三种不同的进口品牌培养基对HEK293细胞进行培养,接种后每隔24小时测定并记录培养液中的细胞密度及细胞活率。结果如下图所示,SMM 293-TI和同类竞争产品相比具有明显的优势。
cell-culture-in-SMM-293-T1
成功表达多种重组蛋白质
SMM 293-TI 成功应用于多种重组蛋白质的表达生产,获得数千个高品质的蛋白和抗体产品。其中一种Fc标签融合蛋白的产量高达2.5g/L。
protein-production-in-SMM-293-T1
已发表文献:

Shen HZ,Zhou Q,Pan XJ,Li ZQ,WuJP,Yan N. Structure of a eukaryotic voltage-gated sodium channel at near-atomic resolution. Science. 2017 Feb 9. doi: 10.1038/nature14892.

Peilong Lu, Xiao-chen Bai, DanMa, Tian Xie. et al. Three-dimensional structure of human γ-secretase. Nature. 2014 Jun 29. doi:10.1038/nature13567.

Xie T, Yan C, Zhou R, , Shi Y, et al. Crystal structure of the γ-secretase component nicastrin. Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13349-54. doi: 10.1073/pnas.1414837111. Epub 2014 Sep 2..

Shangyu D, Shenjie W, Shi Y, et al. Cleavage of amyloid precursor protein by an archaeal presenilin homologue PSH.Proc Natl Acad Sci U S A. 2015 Mar 17; 112(11): 3344–3349.

Sun L, Zhao L, Yang G, Shi Y, et al. Structural basis of human γ-secretase assembly. Proc Natl Acad Sci U S A. 2015 May 12;112(19):6003-8. doi: 10.1073/pnas.1506242112. Epub 2015 Apr 27.

Bai XC, Yan C, Yang G , Shi Y, et al. An atomic structure of human γ-secretase. Nature. 2015 Aug 17. doi: 10.1038/nature14892.

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