Periostin Protein, Human, Recombinant (His Tag)

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Periostin Protein, Human, Recombinant (His Tag): 产品信息

纯度
> 97 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.When cells are added to POSTN-His coated plates (10μg/mL, 100μL/well), approximately >60% will adhere specifically after 30 minutes at 37℃.
蛋白构建
A DNA sequence encoding the human POSTN isoform 3 (NP_001129407.1) corresponding to amino acid (Met 1-Gln 781) was expressed with a C-terminal polyhistidine tag.
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Asn 22
分子量
The recombinant human POSTN consists of 771 amino acids and has a predicted molecular mass of 86 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 80 kDa due to glycosylation.
缓冲液
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Periostin Protein, Human, Recombinant (His Tag): 图片

Periostin Protein, Human, Recombinant (His Tag): 别称

OSF-2 Protein, Human; OSF2 Protein, Human; PDLPOSTN Protein, Human; periostin Protein, Human; PN Protein, Human

Periostin 背景信息

Periostin ( POSTN ), also known as OSF2 (osteoblast specific factor 2), is a heterofunctional secreted extracellular matrix (ECM) protein comprised of four fasciclin domains that promotes cellular adhesion and movement, as well as collagen fibrillogenesis. Postn is expressed in unique growth centers during embryonic development where it facilitates epithelial-mesenchymal transition (EMT) of select cell populations undergoing reorganization. In the adult, Postn expression is specifically induced in areas of tissue injury or areas with ongoing cellular re-organization. In the adult heart Postn is induced in the ventricles following myocardial infarction, pressure overload stimulation, or generalized cardiomyopathy. Although the detailed function of Postn is still unclear, Postn-integrin interaction is thought to be involved in tumor development. Postn is frequently overexpressed in various types of human cancers, stimulating metastatic growth by promoting cancer cell survival, invasion and angiogenesis, and can be a useful marker to predict the behavior of cancer.
全称
periostin, osteoblast specific factor
参考文献
  • Kudo,Y. et al., 2007, Histol Histopathol. 22 (10):1167-1174.
  • Li, J.S. et al., 2007, World J Gastroenterol. 13 (39): 5261-5266.
  • Oku, E. et al., 2008, Int J Hematol. 88 (1): 57-63.
  • Hamilton, D.W. et al., 2008, J Cell Commun Signal. 2(1-2):9-17.
  • Puglisi, F.J et al., 2008, Clin Pathol. 61 (4): 494-498.
  • Conway, S. J. et al., 2008, Curr Genomics. 9 (8): 548-555.
  • Periostin promotes liver fibrogenesis by activating lysyl oxidase in hepatic stellate cells
    Author
    Kumar, P;Smith, T;Raeman, R;Chopyk, DM;Brink, H;Liu, Y;Sulchek, T;Anania, FA;
    Year
    2018
    Journal
    J. Biol. Chem.
    Application
    cell-based
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