PSGL-1 / CD162 Protein, Human, Recombinant (His Tag)

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PSGL-1 / CD162 Protein, Human, Recombinant (His Tag): 产品信息

纯度
(82+15.2) % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Testing in progress
蛋白构建
A DNA sequence encoding the human SELPLG (Q14242) (Met1-Gly295) with a C-terminal polyhistidine tag was expressed.
NCBI Reference No.
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Leu 18
分子量
The recombinant human SELPLG comprises 289 amino acids and has a predicted molecular mass of 30.5 kDa. The apparent molecular mass of the protein is approximately 89 and 61 kDa in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PSGL-1 / CD162 Protein, Human, Recombinant (His Tag): 图片

PSGL-1 / CD162 Protein, Human, Recombinant (His Tag): 别称

CD162 Protein, Human; CLA Protein, Human; PSGL-1 Protein, Human; PSGL1 Protein, Human

PSGL-1 / CD162 背景信息

P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.
全称
selectin P ligand
参考文献
  • 1. Sako, D. et al., 1993, Cell. 75: 1179-1186.
  • 2. Wilkins, P. P. et al., 1995, J. Biol. Chem. 270: 22677-22680.
  • 3. Frenette, P. S. et al., 2000, J. Exp. Med. 191: 1413-1422.
  • 4. Vandendries, E.R .et al., 2004, Thromb. Haemost. 92: 459-466..
  • 5. Pouyani, T. et al., 1995, Cell. 83: 333-343.
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