兔单克隆抗体制备服务

兔单克隆抗体制备服务的特色

兔单克隆抗体通常具有相比鼠单克隆抗体更高的亲和力和特异性,可识别更多新型表位,可识别小鼠抗原等优点,在治疗性候选药物及诊断试剂盒开发中发挥着关键性的作用。义翘神州通过构建抗体库技术开发兔单克隆抗体,直接获得抗体基因及其序列,更有利于优秀抗体的长期保存及抗体外源表达。

更多抗体生产 & 开发服务询价!

兔单克隆抗体制备服务的流程

服务步骤 具体内容 周期 交付内容 交付标准 服务报价

抗原制备询价
详情参阅抗原制备服务相关内容 请填写询价表并发送到
cro-service@sinobiological.com,
我们会尽快与您联系。

抗原复核
• UV 及 SDS-PAGE 分析 1-2天 • 阳性克隆的抗体重链及轻链序列
• 纯化抗体
• 分别含有抗体重链、轻链的商业表达载体
• 实验报告
在ELISA检测中,
纯化抗体与免疫原结合

免疫及血清效价检测
• 免疫前采血
• 2只兔子免疫
• 血清效价检测
• 最终采血
8-10周

建库及筛选
  4-6周

抗体生产及纯化
• 载体构建
• HEK293 细胞瞬转表达
• 蛋白A纯化抗体
2-3周

QC 分析
• SDS-PAGE及UV分析
• ELISA检测
3天
若客户提供抗原(请填写:《客户提供样品信息表》),要求如下:
蛋白抗原 多肽抗原
蛋白总量 2.5-4mg/只兔子 • KLH/VLP 偶联多肽 3-5mg/只兔子
分子量 >10kD 浓度 >0.5mg/mL
电泳纯度 >90% • OVA/Biotin 标记多肽 2-3mg
浓度 >0.5mg/mL 浓度 >0.1mg/mL
缓冲液 缓冲液为 PBS 或经义翘确认对实验无影响的溶液 缓冲液 缓冲液为 PBS 或经义翘确认对实验无影响的溶液

兔单克隆抗体制备服务案例展示

义翘神州建立了高通量兔单克隆抗体研制技术平台,通过此平台自主研发的兔单克隆抗体比传统鼠单克隆抗体具有更好的亲和力和抗原检测限。义翘神州开发的兔单抗可经过 ELISA,WB,IHC,FACS 等多种验证平台,多细胞多组织验证,提高低背景、高灵敏度抗体筛选成功率。

a) 多细胞、多组织验证

  • • Human stomach
    Fig 1. Immunochemical staining of human target E with rabbit Mab. The image showing membrane staining of epithelium cell.
  • • Human rectal cancer
    Fig 2. Immunochemical staining of human target E with rabbit Mab. The image showing membrane staining of epithelium cell in intestinal gland.
  • • Human placenta
    Fig 3. Immunochemical staining of human target E with rabbit Mab. The image showing positive staining of trophoblast.
  • • Human liver
    Fig 4. Immunochemical staining of human target E with rabbit Mab. The image showing membrane staining of hepatocyte.
  • • Human gastric cancer
    Fig 5. Immunochemical staining of human target E with rabbit Mab. The image showing membrane staining of epithelium cell.
     
     
  • • Human esophagus
    Fig 6. Immunochemical staining of human target E with rabbit Mab. The image showing membrane staining of squamous epithelium cell. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen.

b) 多应用验证

  • Fig 1. Immunofluorescence staining of target E in monkey stomach with rabbit Mab. The image showing membrane staining of gastric gland cell. The right panel: merge with DAPI.
  • Fig 2. Immunofluorescence staining of human target E in HeLa cells with rabbit Mab. The image showing membrane staining of HeLa cells.
  • Fig 3. Lane A: A431 Whole Cell Lysate. Anti-target E rabbit Mab; Goat Anti-Rabbit IgG H&L (Dylight800).
  • Fig 4. Lane A: K562 Whole Cell Lysate. Anti-target E rabbit Mab; Clean-Blotô IP Detection Reagent (HRP).
  • Fig 5. Analysis of anti-target E reactivity on HeLa cells.
     

a) 多细胞、多组织验证

  • • Human mammary gland
    Fig 1. Immunochemical staining of human target F with rabbit Mab. Positive staining was localized to membrane of alveolus epithelium.
  • • Human colon carcinoma
    Fig 2. Immunochemical staining of human target F with rabbit Mab. Positive staining was localized to membrane of colonic gland epithelium.
  • • Human bladder carcinoma
    Fig 3. Immunochemical staining of human target F with rabbit Mab. Positive staining was localized to membrane of transitional epithelium.

b) 多应用验证

  • Fig 1. Immunofluorescence staining of human target F in SKBR3 cells. Positive staining was localized to plasma membrane.
     
     
     
  • Fig 2. Lane A: MCF-7 Whole Cell Lysate. Anti-target F rabbit Mab; Goat Anti-Rabbit IgG H&L (Dylight800).
     
     
     
     
  • Fig 3. Flow cytometric analysis of anti-target F reactivity on SKBR3 cells. The histogram were derived from the gated events based on light scattering characteristics of viable cells.
     
     
  • Fig 4. Lane A: MCF-7 Whole Cell Lysate; Lane B: A431 Whole Cell Lysate; Lane C: HepG2 Whole Cell Lysate; Lane D: Caco-2 Whole Cell Lysate. Anti-target F rabbit Mab; Dylight 800-labeled antibody to rabbit IgG (H+L).

义翘神州第二代兔单抗技术及优势

第二代制备技术 液相+固相筛选 多种验证平台精选最优抗体
  • 高亲和力
  • 识别鼠源蛋白抗体
  • 高特异性和低本底
  • 易于人源化
  • 可识别更多新型表位
  • 第二代兔单抗可直接用于基因工程改造
  • 抗体库更大

兔单克隆抗体制备服务的优势

  • 国际领先的蛋白重组表达生产及纯化技术,多种蛋白表达及纯化平台,成功表达 >6000 蛋白经验,抗原表达成功率高
  • 专业高效的多肽设计软件及高效偶联方法,保证多肽免疫成功率 95% 以上
  • 业界领先的抗体库构建及筛选技术,减少抗体片段缺失或错配的风险,提高筛选到目标抗体的概率
  • ELISA,WB,IHC,FACS 等多种验证平台,多细胞多组织验证,提高低背景、高灵敏度抗体筛选成功率
  • 亲和力高,平均超过鼠单抗 10-100 倍
  • 从抗体开发,载体构建到抗体表达纯化一站式服务,节省客户时间
  • 分步报价,按节点收费,全面降低客户风险,节约成本

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