TNF-alpha Protein, Human, Recombinant

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TNF-alpha Protein, Human, Recombinant: 产品信息

纯度
> 95 % as determined by SDS-PAGE
内毒素
< 0.01 EU per μg of the protein as determined by the LAL method
生物活性
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 5-50pg/mL.The specific activity of Recombinant Human TNF-alpha is approximately 52000 IU/μg,which is calibrated against human TNF-alpha WHO International Standard(NIBSC code:12/154).
蛋白构建
A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val77-Leu233) was expressed with an initial Met at the N-terminus.
NP No.
表达宿主
E. coli
种属
Human
预测 N 端
Met
分子量
The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa.
缓冲液
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TNF-alpha Protein, Human, Recombinant: 图片

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 5-50pg/mL.The specific activity of Recombinant Human TNF-alpha is approximately 46000IU/μg,which is calibrated against human TNF-alpha WHO International Standard(NIBSC code:12/154).

TNF-alpha Protein, Human, Recombinant: 别称

DIF Protein, Human; TNF-alpha Protein, Human; TNFA Protein, Human; TNFSF2 Protein, Human

TNF-alpha 背景信息

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD12a; p55/6) and TNF-R2 (TNF receptor type 2; CD12b; p75/8), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
全称
tumor necrosis factor
参考文献
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
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