血管紧张素转换酶2(ACE2)

血管紧张素转换酶2(ACE2)是气道上皮细胞表面表达的一种膜受体。在21世纪初,科学家开始对ACE2进行深入的研究。ACE2被证实是SARS-COVHCoV-NL63这两种人冠状病毒的宿主细胞受体,可以与冠状病毒的Spike蛋白相互作用。这种相互作用由Spike蛋白的RBD结构域介导,并且被认为是病毒与细胞进行膜融合的关键步骤。最近,ACE2又再一次引起人们的关注。研究表明,ACE2是新发现的一种致命性新型冠状病毒SARS-CoV-2(2019-nCoV)的宿主细胞受体。该发现表明阻断S蛋白和ACE2之间的相互作用是治疗冠状病毒感染的有效方法。

从理论上讲,SARS-CoV-2病毒以及受体ACE2均可作为治疗性靶点对病毒感染进行有效的干预。可溶性Spike蛋白或阻断抗体都可以用来封闭ACE2,从而防止病毒感染。同样的,可溶的ACE2-Fc融合蛋白可用于封闭病毒体上的S1蛋白,从而达到相同的效果。

此外,除了在介导病毒进入宿主细胞过程中发挥重要作用外,ACE2极可能在呼吸系统疾病的发病机理中也发挥着举足轻重的作用。ACE2是肾素-血管紧张素系统(RAS)的重要调节因子。ACE2可以通过将血管紧张素II转化为产物血管紧张素1-7而负调节其活性。在由RSV和禽流感(H5N1)感染引起的肺损伤中可以观察到ACE2的失调,并且在动物模型中也已显示ACE2-Fc融合蛋白的使用可以减轻症状。

义翘神州研发生产了一系列ACE2相关的蛋白、抗体试剂。其中,ACE2蛋白试剂可同时具有结合spike的活性以及酶活性。

Cat#   Species More
  Human

Expressed Host HEK293
Tag His
Activity Bind with SARS-CoV-2 Spike RBD
  Human

Expressed Host HEK293
Tag Fc
Activity  
  Human

Expressed Host HEK293
Tag mFc
Activity  
  Rhesus

Expressed Host HEK293
Tag Fc
Activity Bind with SARS-CoV-2 Spike RBD; enzymatic activity
  Rhesus

Expressed Host HEK293
Tag His
Activity enzymatic activity
  Rat

Expressed Host HEK293
Tag His
Activity enzymatic activity
  Mouse

Expressed Host HEK293
Tag His
Activity enzymatic activity
  Mouse

Expressed Host HEK293
Tag His & Fc
Activity  
  Mouse

Expressed Host HEK293
Tag His
Activity  
Cat#   Species More
  Human

Ab Type Rabbit PAb
Application ELISA, IHC-P
  Human

Ab Type Rabbit PAb
Application WB, ELISA, IHC-P
  Mouse

Ab Type Rabbit PAb
Application ELISA
  Mouse

Ab Type Rabbit PAb
Application ELISA, IHC-P
  Rat

Ab Type Mouse MAb
Application ELISA(Cap)
  Rat

Ab Type Rabbit MAb
Application ELISA
Cross-reactivity Human, Mouse, Rhesus
  Rat

Ab Type Rabbit MAb
Application ELISA(Det)
Cross-reactivity Human, Mouse, Rhesus
  Rat

Ab Type Rabbit MAb
Application IHC-P
Cross-reactivity Mouse
  Rat

Ab Type Rabbit PAb
Application ELISA
Cross-reactivity Human, Mouse, Rhesus
  Rat

Ab Type Rabbit PAb
Application ELISA
Cross-reactivity Human, Mouse, Rhesus
 

参考文献

• Jiang F.Angiotensin-converting enzyme 2 and angiotensin 1-7: novel therapeutic targets.Nat Rev Cardiol. 2014
• Williams VR.Angiotensin-converting enzyme 2 and renal disease.Curr Opin Nephrol Hypertens. 2018
• Jia H.Pulmonary Angiotensin-Converting Enzyme 2 (ACE2) and Inflammatory Lung Disease.Shock. 2016