Anti-Neuroserpin Antibody

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Anti-Neuroserpin Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-Neuroserpin Antibody
经验证的应用
WB,ELISA,IHC-P
交叉反应
Reacts with: Human
特异性
Human Neuroserpin
免疫原
Recombinant Human SerpinI1 protein (Catalog#11107-H08H)
制备方法
Produced in rabbits immunized with purified, recombinant Human SerpinI1 (rh SerpinI1; Catalog#11107-H08H; NP_005016.1; Met 1-Leu 410). SerpinI1 specific IgG was purified by Human SerpinI1 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Neuroserpin Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:1000-1:2000
IHC-P 1:500-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Neuroserpin Antibody (Rabbit Polyclonal 抗体) 图片

Immunochemical staining of human SerpinI1 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Anti-SERPINI1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Mouse brain Tissue Lysate

Lane B: Rat brain Tissue Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:46 kDa

Observed band size:52 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Neuroserpin Antibody: 别称

Anti-neuroserpin Antibody; Anti-PI12 Antibody; Anti-Serpin I1 Antibody

Neuroserpin 背景信息

Neuroserpin, also known as Protease inhibitor 12 and SERPINI1, is a secreted protein which belongs to the serpin family. Neuroserpin is a serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. Serine protease inhibitors of the serpin superfamily are involved in many cellular processes. Neuroserpin was first identified as a protein secreted from the axons of dorsal root ganglion neurons. Neuroserpin is predominantly expressed in the brain, and is expressed in the late stages of neurogenesis during the process of synapse formation. Overexpression of neuroserpin in an anterior pituitary corticotroph cell line results in the extension of neurite-like processes, suggesting that neuroserpin may play a role in cell communication, cell adhesion, and/or cell migration. Neuroserpin may be involved in the formation or reorganization of synaptic connections, as well as synaptic plasticity in the adult nervous system. Neuroserpin may also protect neurons from cell damage by tissue-type plasminogen activator. Defects of neuroserpin are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B).
全称
serpin peptidase inhibitor, clade I (neuroserpin), member 1
参考文献
  • Schrimpf SP. et al., 1997, Genomics. 40 (1): 55-62.
  • Hill RM. et al., 2002, Ann N Y Acad Sci. 971: 406-15.
  • Yepes M. et al., 2004, Thromb. Haemost. 91 (3): 457-64.
  • Galliciotti G. et al., 2006, Front Biosci. 11: 33-45.
  • Neuroserpin expression during human brain development and in adult brain revealed by immunohistochemistry and single cell RNA sequencing
    Author
    Adorjan, I;Tyler, T;Bhaduri, A;Demharter, S;Finszter, CK;Bako, M;Sebok, OM;Nowakowski, TJ;Khodosevich, K;Møllgård, K;Kriegstein, AR;Shi, L;Hoerder-Suabedissen, A;Ansorge, O;Molnár, Z;
    Year
    2019
    Journal
    J. Anat.
    Application
    IHC
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