Anti-TNF-alpha Antibody

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Anti-TNF-alpha Antibody (Mouse Monoclonal 抗体) 产品信息

产品名称
Anti-TNF-alpha Antibody
经验证的应用
ELISA,ELISA(Cap),FCM
交叉反应
Reacts with: Human
特异性
Human TNF-alpha
免疫原
Recombinant Human TNFα protein (Catalog#10602-HNAE)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Tumor necrosis factor-alpha (rhTNFα; Catalog#10602-HNAE; Val 77-Leu 233; NP_000585.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #9A7A5G9
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-TNF-alpha Antibody (Mouse Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
ELISA 1:1000-1:2000
FCM 1:25-1:100
ELISA(Cap) 1:250-1:2000
Notes
ELISA(Cap): This antibody will detect Human TNF-alpha / TNFA / TNFSF2 in ELISA pair set (Catalog: # SEKA10602). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 10602-MM08).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-TNF-alpha Antibody (Mouse Monoclonal 抗体) 图片

Flow cytometric analysis of Purified anti-Human TNFa on CHO transfected cells and stimulated lymphocytes. Left panel The transfected cells stained with Purified Mouse anti-TNFa (Bold line hisgram), To demonstrate specificity of staining, the binding of 10602-MM01 was blocked by the preincubation of the purified antibody with molar excess of recombinant human TNFa (5 μg, Cat. No. 10602-HNAE) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody. Right panel Human PBMC were stimulated for 4 hrs with Leukocyte Activation Cocktail (with BD GolgiPlug™, BD Pharmingen™ Cat. No. 550583), then cells were harvested and treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714).

Anti-TNF-alpha Antibody: 别称

Anti-DIF Antibody; Anti-TNF-alpha Antibody; Anti-TNFA Antibody; Anti-TNFSF2 Antibody

TNF-alpha 背景信息

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD12a; p55/6) and TNF-R2 (TNF receptor type 2; CD12b; p75/8), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
全称
tumor necrosis factor
研究领域
参考文献
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • Approaching near real-time biosensing: microfluidic microsphere based biosensor for real-time analyte detection
    Author
    Cohen, N;Sabhachandani, P;Golberg, A;Konry, T;
    Year
    2015
    Journal
    Biosens Bioelectron
  • Microsphere-based immunoassay integrated with a microfluidic network to perform logic operations
    Author
    Sabhachandani, P;Cohen, N;Sarkar, S;Konry, T;
    Year
    2015
    Journal
    Microchimica Acta
    Application
    anti-TNF-α antibody detection with microsphere sensors
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