Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 protein (His Tag)

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Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 protein (His Tag): 产品信息

纯度
> 95 % as determined by SDS-PAGE.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
生物活性
Testing in progress
蛋白构建
A DNA sequence encoding the Zika virus (strain Zika SPH2015) NS1 (ALU33341.1) (Val796-Leu1157) was expressed with a polyhistidine tag at the N-terminus.
GenBank No.
表达宿主
HEK293 Cells
种属
ZIKV
预测 N 端
His
分子量
The recombinant Zika virus (strain Zika SPH2015) NS1 consists of 381 amino acids and predicts a molecular mass of 43.5 kDa.
缓冲液
Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 protein (His Tag): 图片

ZIKV Nonstructural protein 1 背景信息

Zika virus NS1 antigen is one of seven non-structural proteins. NS1 is involved in RNA replicaiton. The possible effects of NS1 on hosts include: localization to host cell surface and secreted extracellularly, modelates signalling of innate immune system, has possible damages to platelets and endothelial cells through anti-NS1 antibodies.
参考文献
    1. Wong SS-Y, et al., Zika virus infectiondthe next wave after dengue?, Journal of the Formosan Medical Association (2016)
  • Deciphering Protein O-Glycosylation: Solid-Phase Chemoenzymatic Cleavage and Enrichment
    Author
    Yang, S;Onigman, P;Wu, WW;Sjogren, J;Nyhlen, H;Shen, RF;Cipollo, J;
    Year
    2018
    Journal
    Anal. Chem.
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