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14-3-3 beta / YWHAB 抗体, 鼠单抗

  • Human CD69 / CLEC2C Immunoprecipitation(IP) 8059
  • 14-3-3 beta / YWHAB Antibody, Mouse MAb, Western blot
  • 14-3-3 beta / YWHAB Antibody, Mouse MAb, Immunoprecipitation
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食蟹猴 YWHAB 抗体产品信息
免疫原:Recombinant Cynomolgus 14-3-3 beta / YWHAB protein (Catalog#90022-CNCE)
克隆号:05
Ig 类型:Mouse IgG2b
浓度:
内毒素:
缓冲液:0.2 μm filtered solution in PBS with 5% trehalose
抗体制备方法:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus 14-3-3 beta / YWHAB (Catalog#90022-CNCE; Q4R572-2; Met 2-Asn 244). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
食蟹猴 YWHAB 抗体WB实验图片
食蟹猴 YWHAB 抗体IP实验图片
Other YWHAB 抗体 Products

Anti-Histone H3 rabbit monoclonal antibody at 1:200 dilution

Lane A: NIH3T3 Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:15 kDa

Observed band size:17 kDa

14-3-3 beta/YWHAB 研究背景

14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.

食蟹猴 14-3-3 beta/YWHAB 参考资料
  • Tommerup N, et al. (1996) Assignment of the human genes encoding 14,3-3 Eta (YWHAH) to 22q12, 14-3-3 zeta (YWHAZ) to 2p25.1-p25.2, and 14-3-3 beta (YWHAB) to 20q13.1 by in situ hybridization. Genomics. 33(1): 149-50.
  • Jin YH, et al. (2008) Sirt2 interacts with 14-3-3 beta/gamma and down-regulates the activity of p53. Biochem Biophys Res Commun. 368(3): 690-5.
  • Sekimoto T, et al. (2004) 14-3-3 suppresses the nuclear localization of threonine 157-phosphorylated p27(Kip1). EMBO J. 23(9): 1934-42.
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    货号: 90022-MM05-50
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