Anti-14-3-3 sigma Antibody

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Anti-14-3-3 sigma Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-14-3-3 sigma Antibody
经验证的应用
WB,ELISA,IHC-P,ICC/IF,IP
交叉反应
Reacts with: Mouse
特异性
Mouse 14-3-3 sigma
免疫原
Recombinant Mouse 14-3-3 sigma/SFN Protein (Catalog#50576-MNCE)
制备方法
Produced in rabbits immunized with purified, recombinant Mouse 14-3-3 sigma/SFN (rM 14-3-3 sigma/SFN; Catalog#50576-MNCE; NP_061224.2; Met1-Ser248). 14-3-3 sigma/SFN specific IgG was purified by Mouse 14-3-3 sigma/SFN affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-14-3-3 sigma Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:2000-1:20000
ICC-IF 1:100-1:500
IP IP:1-5μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-14-3-3 sigma Antibody (Rabbit Polyclonal 抗体) 图片

Anti-SFN rabbit polyclonal antibody at 1:500 dilution

Lane A: NIH3T3 Whole Cell Lysate

Lane B: Raw 264.7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:27 kDa

Immunochemical staining of mouse SFN in mouse skin with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of mSFN in Raw264.7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Mouse mSFN polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.

Mouse SFN was immunoprecipitated using:

Lane A:0.5 mg NIH3T3 Whole Cell Lysate

4 µL anti-Mouse SFN rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-Mouse SFN rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 30 kDa

Observed band size :30 kDa

Anti-14-3-3 sigma Antibody: 别称

Anti-Er Antibody; Anti-Mme1 Antibody; Anti-Ywhas Antibody

14-3-3 sigma 背景信息

14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 1 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.
全称
stratifin
参考文献
  • Zakut-Houri R., et al.,(1985), Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4:1251-1255.
  • Lamb P., et al., (1986), Characterization of the human p53 gene.Mol. Cell. Biol. 6:1379-1385.
  • Harlow E., et al.,(1985), Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.Mol. Cell. Biol. 5:1601-1610.
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