Anti-DYKDDDDK (FLAG® epitope Tag) Antibody

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Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal 抗体) 产品信息

产品名称
Anti-DYKDDDDK (FLAG® epitope Tag) Antibody
经验证的应用
ICC/IF,IF
特异性
DYKDDDDK (FLAG® epitope Tag)
免疫原
A synthetic peptide corresponding to the flag-tag sequence (DYKDDDDK).
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, the synthetic peptide corresponding to the DYKDDDDK-tag sequence (CDYKDDDDK). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG2b Clone #01
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:50-1:1000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal 抗体) 图片

Immunofluorescence staining of flag-Tag in 293 cells, transfected with PCMV-CDH1-flag (Figure A), pSTEP2-Flag-FABP4-GST (Figure B), pSTEP2-Flag-ARG1-GST (Figure C), Flag-PRMT5-His (Figure D),Flag-PRMT6-His (Figure E) and His-flag-CD38(Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-flag-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
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