Anti-E2F4 Antibody

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Anti-E2F4 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-E2F4 Antibody
经验证的应用
WB,ICC/IF
交叉反应
Reacts with: Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
特异性
Human E2F4
免疫原
A synthetic peptide corresponding to the center region of the Human E2F4
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human E2F4, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
PBS, pH7.0 with 0.03% Proclin300
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-E2F4 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ICC-IF 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-E2F4 Antibody (Rabbit Polyclonal 抗体) 图片

Anti-E2F4 rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lane B: Raji Whole Cell Lysate

Lane C: HeLa Whole Cell Lysate

Lane D: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:44 kDa

Observed band size:53 kDa

Immunofluorescence staining of E2F4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human E2F4 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

Anti-E2F4 Antibody: 别称

Anti-E2F-4 Antibody; Anti-E2F4 Antibody
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