Anti-Galectin 7 Antibody

1/3
Price:
Size:
Number:

Anti-Galectin 7 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-Galectin 7 Antibody
经验证的应用
WB,IHC-P,ICC/IF
交叉反应
Reacts with: Human
特异性
Human Galectin 7
免疫原
Recombinant Human Galectin 7/LGALS7 Protein (Catalog#12000-H07E)
制备方法
Produced in rabbits immunized with purified, recombinant Human Galectin 7/LGALS7 (rh Galectin 7/LGALS7; Catalog#12000-H07E; NP_002298.1; Ser2-Phe136). Galectin 7/LGALS7 specific IgG was purified by Human Galectin 7/LGALS7 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Galectin 7 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
IHC-P 1:2500-1:10000
ICC-IF 1:1500-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Galectin 7 Antibody (Rabbit Polyclonal 抗体) 图片

Anti-LGALS7 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: MDA-MB-231 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:15 kDa

Observed band size:15 kDa

(We are unsure as to the identity of these extra bands.)

Immunochemical staining of human LGALS7 in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Immunofluorescence staining of LGALS7 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human LGALS7 polyclonal antibody (dilution ratio 1:5000) at 4℃ overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.

Anti-Galectin 7 Antibody: 别称

Anti-GAL7 Antibody; Anti-LGALS7A Antibody

Galectin 7 背景信息

LGALS7, also known as Galectin-7, is a member of the galectins family. The galectins are a family of beta-galactoside-binding proteins. There are at least 14 identified members in this family. Galectins share similarities in the CRD (the carbohydrate recognition domain). They are synthesized as cytosolic proteins. Though localized principally in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or alternatively targeted to the nucleus. Galectins are implicated in modulating cell-cell and cell-matrix interactions. LGALS7 contains 1 galectin domain and is mainly expressed in stratified squamous epithelium. Galectin-7 could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. LGALS7 is a pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.
全称
lectin, galactoside-binding, soluble, 7
参考文献
  • Villeneuve C, et al. (2011) Mitochondrial proteomic approach reveals galectin-7 as a novel BCL-2 binding protein in human cells. Mol Biol Cell. 22(7):999-1013.
  • Rondanino C, et al. (2011) Galectin-7 modulates the length of the primary cilia and wound repair in polarized kidney epithelial cells. Am J Physiol Renal Physiol. 301(3):F622-33.
  • Masuyer G, et al. (2012) Inhibition mechanism of human galectin-7 by a novel galactose-benzylphosphate inhibitor. FEBS J. 279(2):193-202.
  • Magnaldo T, et al. (1995) Galectin-7, a human 14-kDa S-lectin, specifically expressed in keratinocytes and sensitive to retinoic acid. Dev Biol. 168(2):259-71.
  • Madsen P, et al. (1995) Cloning, expression, and chromosome mapping of human galectin-7. J Biol Chem. 270(11):5823-29.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后