Anti-PGK2 Antibody

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Anti-PGK2 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-PGK2 Antibody
经验证的应用
WB,IP
交叉反应
Reacts with: Human
Predicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology)
特异性
Human PGK2
免疫原
A synthetic peptide corresponding to the center region of the Human PGK2
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PGK2, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-PGK2 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IP IP:1-5 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PGK2 Antibody (Rabbit Polyclonal 抗体) 图片

Anti-PGK2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: HeLa Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:45 kDa

Observed band size:45 kDa

PGK2 was immunoprecipitated using:

Lane A:0.5 mg HeLa Whole Cell Lysate

4 µL anti-PGK2 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-PGK2 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 45 kDa

Observed band size :45 kDa

Anti-PGK2 Antibody: 别称

Anti-dJ417L20.2 Antibody; Anti-HEL-S-272 Antibody; Anti-PGKB Antibody; Anti-PGKPS Antibody

PGK2 背景信息

全称
phosphoglycerate kinase 2
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