Anti-Troponin C Antibody

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Anti-Troponin C Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-Troponin C Antibody
经验证的应用
ELISA,IHC-P,IP
交叉反应
Reacts with: Human, Cynomolgus
特异性
Human Troponin C
免疫原
Recombinant Human Troponin C/TNNC1 Protein (Catalog#11011-HNAE)
制备方法
Produced in rabbits immunized with purified, recombinant Human Troponin C/TNNC1 (rh Troponin C/TNNC1; Catalog#11011-HNAE; NP_003271.1; Met1-Glu161). Troponin C/TNNC1 specific IgG was purified by Human Troponin C/TNNC1 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Troponin C Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
ELISA 1:5000-1:10000
IHC-P 1:500-1:2000
IP IP:5-10 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Troponin C Antibody (Rabbit Polyclonal 抗体) 图片

Immunochemical staining TNNC1 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining TNNC1 in cynomolgus heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

TNNC1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

4 µL anti-TNNC1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-TNNC1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size :16 kDa

Anti-Troponin C Antibody: 别称

Anti-CMD1Z Antibody; Anti-CMH13 Antibody; Anti-TN-C Antibody; Anti-TNC Antibody; Anti-TNNC Antibody

Troponin C 背景信息

Troponin I, also known as TNNC1, is part of the troponin complex. This complex contains 3 subunits: troponin I (TnI), troponin T (TnT) and troponin C (TnC). Troponin I is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle.
全称
troponin C type 1 (slow)
参考文献
  • Kalaji FR. et al., 2012, Saudi J Kidney Dis Transpl. 23 (5): 939-45.
  • Wijnker PJ. et al., 2013, Am J Physiol Heart Circ Physiol. 304 (2): H260-8.
  • Solaro RJ. et al., 2013, Circ Res. 112 (2): 355-66.
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