Anti-V5 tag Antibody

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Anti-V5 tag Antibody (Mouse Monoclonal 抗体) 产品信息

产品名称
Anti-V5 tag Antibody
经验证的应用
WB,IP
特异性
V5 tag
免疫原
A synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST).
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #01
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-V5 tag Antibody (Mouse Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IP 1-4 μg/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-V5 tag Antibody (Mouse Monoclonal 抗体) 图片

Anti-V5 Tag mouse monoclonal antibody at 1:500 dilution.

Lane A: his-ARG1-V5 transfected 293 cell Lysate

(60ug)

Lane B: mFABP4-V5-his transfected 293 cell Lysate

(60ug)

Lane C: V5-ARG1-his transfected 293 cell Lysate

(10ug)

Lane D: V5-mFABP4-his transfected 293 cell Lysate

(20ug)

Lane E: Negative control transfected 293 cell Lysate

(30ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions

V5 tag was immunoprecipitated using:

Lane A:0.5 mg V5-ARG1-His transfected 293 Whole Cell Lysate

Lane B:0.5 mg V5-mFABP4-His transfected 293 Whole Cell Lysate

2 µL anti-V5 tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-V5 tag mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: kDa

Observed band size: kDa

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