Anti-V5 tag Antibody

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Anti-V5 tag Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-V5 tag Antibody
经验证的应用
WB,IP
特异性
V5 tag
免疫原
A synthetic peptide corresponding to the V5 tag sequence.
制备方法
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the V5-tag sequence. V5-tag specific IgG was purified by V5-taq affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-V5 tag Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:1000-1:5000
IP 1-4 μl/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-V5 tag Antibody (Rabbit Polyclonal 抗体) 图片

V5-tag was immunoprecipitated using:

Lane A:0.5 mg his-ARG1-V5 Transfected 293 Cell Lysate

Lane B:0.5 mg V5-ARG1-his Transfected 293 Cell Lysate

Lane C:0.5 mg mFABP4-V5-his Transfected 293 Cell Lysate

Lane D:0.5 mg V5-mFABP4-his Transfected 293 Cell Lysate

Lane E:0.5 mg Negative control

4 μL anti-V5-tag rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-V5-tag rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-V5 Tag rabbit polyclonal antibody at 1:1000 dilution

Sample:Recombinant Protein

Lane A: V5-mFABP4-his(30ng)

Lane B: his-ARG1-V5(30ng)

Lane C: mFABP4-V5-his(30ng)

Lane D:Negative control

Secondary

Dylight 800-labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-V5 Tag rabbit polyclonal antibody at 1:1000 dilution

Lane A: his-ARG1-V5 Transfected 293 Cell Lysate

Lane B: V5-ARG1-his Transfected 2933 Cell Lysate

Lane C: V5-mFABP4-his Transfected 293 Cell Lysate

Lane D: mFABP4-V5-his Transfected 293 Cell Lysate

Lane E: Negative control

Lysates/proteins at 2 μg per Lane.

Secondary

Dylight 800-labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

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