Beta-Tubulin Loading Control Antibody, Mouse MAb

Beta-Tubulin Loading Control Antibody, Mouse MAb (Mouse Monoclonal 抗体) 产品信息

产品名称
Beta-Tubulin Loading Control Antibody, Mouse MAb
经验证的应用
WB,IHC-P,ICC/IF,IF,IP
交叉反应
Reacts with: Human
特异性
Human Beta-Tubulin
免疫原
A synthetic peptide corresponding to the N-terminus of the human beta-tubulin
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG2b Clone #05
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Beta-Tubulin Loading Control Antibody, Mouse MAb (Mouse Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1/1000-1/100000
IHC-P 1/2000-1/20000
ICC-IF 1/50-1/200
IP IP:5-10 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Beta-Tubulin Loading Control Antibody, Mouse MAb (Mouse Monoclonal 抗体) 图片

Immunochemical staining of human Tubulin in human breast carcinoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human Tubulin in human malignant melanoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human Tubulin in human prostate with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
Immunofluorescence staining of Human Beta-Tubulin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human Beta-Tubulin monoclonal antibody at 4℃ overnight. Then cells were stained with the Alexa Fluor® 594-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®488-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoskeleton.

Anti-Beta-Tubulin mouse monoclonal antibody at 1:10000, 1:20000, 1:50000, 1:100000 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lane C: Raw246.7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:54 kDa

Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution

Lane A: Rabbit heart tissue lysate

Lane B: Rabbit liver tissue lysate

Lane C: Rabbit spleen tissue lysate

Lane D: Rabbit lung tissue lysate

Lane E: Rabbit kidney tissue lysate

Lane F: Rabbit brain tissue lysate

Lane G: Rabbit muscle tissue lysate

Lane H: Rabbit stomach tissue lysate

Lane I: Rabbit small intestine tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:54 kDa

Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution

Lane A: Rat heart tissue lysate

Lane B: Rat liver tissue lysate

Lane C: Rat spleen tissue lysate

Lane D: Rat lung tissue lysate

Lane E: Rat kidney tissue lysate

Lane F: Rat muscle tissue lysate

Lane G: Rat stomach tissue lysate

Lane H: Rat small intestine tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:54 kDa

Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution

Lane A: Mouse heart tissue lysate

Lane B: Mouse liver tissue lysate

Lane C: Mouse spleen tissue lysate

Lane D: Mouse lung tissue lysate

Lane E: Mouse kidney tissue lysate

Lane F: Mouse brain tissue lysate

Lane G: Mouse muscle tissue lysate

Lane H: Mouse stomach tissue lysate

Lane I: Mouse pancreas tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:54 kDa

Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: K562 Whole Cell Lysate

Lane D: Hela Whole Cell Lysate

Lane E: RAW264.7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:54 kDa

Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: Daudi Whole Cell Lysate

Lane C: MOLT-4 Whole Cell Lysate

Lane D: A549 Whole Cell Lysate

Lane E: 293T Whole Cell Lysate

Lane F: HelaS3 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:54 kDa

Beta-Tubulin 背景信息

Beta-Tubulin is a subunit of tubulin. Tubulin is one of several members of a small family of globular proteins. It is the major constituent of microtubules. There are two most common members of the tubulin family: alpha-tubulin and beta-tubulin, and together their dimers form microtubules. The dimers of alpha- and beta-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the beta -tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament. Beta-tubulin faces the plus end of the microtubule while alpha-tubulin faces the minus end. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are examining. This ensures constant expression levels. Thus “housekeeping genes” are frequently chosen for use as loading controls. It is also important that the protein chosen as a loading control has a different molecular weight than the protein of interest so that the bands are distinct and expression levels quantifiable. Popular loading control detection antibodies include anti-β-Actin monoclonal or polyclonal antibodies, anti-COX-4, anti-GAPDH, anti-Tubulin and anti-VDAC/Porin antibodies.
参考文献
  • Williams RC Jr. et al., 1999. Anal Biochem. 275(2): 265-7.
  • Nogales E. et al., 1998. Nat Struct Biol. 5(6): 451-8.
  • Dutcher SK. 2001. Curr Opin Cell Biol. 13(1): 49-54.
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