Anti-Nectin-2 Antibody

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Anti-Nectin-2 Antibody (Rabbit Monoclonal 抗体) 产品信息

产品名称
Anti-Nectin-2 Antibody
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Mouse
特异性
Mouse Nectin-2
No cross-reactivity in ELISA with
Human CD112 / Nectin-2
免疫原
Recombinant Mouse PVRL2 protein (Catalog#50318-M08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse PVRL2 extracellular domain (rM PVRL2; Catalog#50318-M08H; NP_033016.3; Met 1-Gly 351).
来源
Monoclonal Rabbit IgG Clone #110
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Nectin-2 Antibody (Rabbit Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:5000-1:10000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Nectin-2 Antibody (Rabbit Monoclonal 抗体) 图片

Anti-mPVRL2 rabbit monoclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:58 kDa

Observed band size:58 kDa

(We are unsure as to the identity of these extra bands.)

Mouse PVRL2 was immunoprecipitated using:

Lane A:0.5 mg Raji Whole Cell Lysate

0.5 µL anti-Mouse PVRL2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse PVRL2 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 58 kDa

Observed band size: 58 kDa

Anti-Nectin-2 Antibody: 别称

Anti-AI325026 Antibody; Anti-AI987993 Antibody; Anti-Cd112 Antibody; Anti-MPH Antibody; Anti-nectin-2 Antibody; Anti-Pvr Antibody; Anti-Pvs Antibody

Nectin-2 背景信息

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
全称
poliovirus receptor-related 2 (herpesvirus entry mediator B)
参考文献
  • Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
  • Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
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