Anti-CD4 Antibody

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Anti-CD4 Antibody (Rabbit Monoclonal 抗体) 产品信息

产品名称
Anti-CD4 Antibody
经验证的应用
ELISA,IHC-P
交叉反应
Reacts with: Mouse
特异性
Mouse CD4
No cross-reactivity in ELISA with
Human CD4
免疫原
Recombinant Mouse CD4 protein (Catalog#50134-M08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD4 (rM; Catalog#50134-M08H; NP_038516.1; Met 1-Thr 394).
来源
Monoclonal Rabbit IgG Clone #1
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD4 Antibody (Rabbit Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
ELISA 1:5000-1:10000
IHC-P 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD4 Antibody (Rabbit Monoclonal 抗体) 图片

Immunochemical staining of mouse CD4 in mouse spleen (from three donors) with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

Anti-CD4 Antibody: 别称

Anti-L3T4 Antibody; Anti-Ly-4 Antibody

CD4 背景信息

T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. All human tissue sources of CD4 bind radiolabeled gp12 to the same relative degree; however, the murine homologous protein, L3T4, does not bind the HIV envelope protein. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. The CD4 molecule is currently the object of intense interest and investigation both because of its role in normal T-cell function, and because of its role in HIV infection. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Viral protein U (VpU) of HIV-1 plays an important role in downregulation of the main HIV-1 receptor CD4 from the surface of infected cells. Physical binding of VpU to newly synthesized CD4 in the endoplasmic reticulum is an early step in a pathway leading to proteasomal degradation of CD4. Amino acids in both helices found in the cytoplasmic region of VpU in membrane-mimicking detergent micelles experience chemical shift perturbations upon binding to CD4, whereas amino acids between the two helices and at the C-terminus of VpU show no or only small changes, respectively. Paramagnetic spin labels were attached at three sequence positions of a CD4 peptide comprising the transmembrane and cytosolic domains of the receptor. VpU binds to a membrane-proximal region in the cytoplasmic domain of CD4.
全称
CD4 molecule
参考文献
  • Farrar WL, et al. (1988) Characterization of CD4 glycoprotein determinant-HIV envelope protein interactions: perspectives for analog and vaccine development. Crit Rev Immunol. 8(4): 315-39.
  • Biddison WE, et al. (1989) CD4 expression and function in HLA class II-specific T cells. Immunol Rev. 109: 5-15.
  • Singh SK, et al. (2012) Mapping the interaction between the cytoplasmic domains of HIV-1 viral protein U and human CD4 with NMR spectroscopy. FEBS J. 279(19):3705-14.
  • TGFβ drives immune evasion in genetically reconstituted colon cancer metastasis
    Author
    Tauriello, DVF;Palomo-Ponce, S;Stork, D;Berenguer-Llergo, A;Badia-Ramentol, J;Iglesias, M;Sevillano, M;Ibiza, S;Cañellas, A;Hernando-Momblona, X;Byrom, D;Matarin, JA;Calon, A;Rivas, EI;Nebreda, AR;Riera, A;Attolini, CS;Batlle, E;
    Year
    2018
    Journal
    Nature
    Application
    IHC
  • The Cdx2 homeobox gene suppresses intestinal tumorigenesis through non-cell-autonomous mechanisms
    Author
    Balbinot, C;Armant, O;Elarouci, N;Marisa, L;Martin, E;De Clara, E;Onea, A;Deschamps, J;Beck, F;Freund, JN;Duluc, I;
    Year
    2018
    Journal
    J. Exp. Med.
    Application
    IHC
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