Recombinant Mouse CD80/B7-1 Protein (Catalog#50446-M08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD80/B7-1 (rM CD80/B7-1; Catalog#50446-M08H; Q00609-1; Met1-Lys245) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Monoclonal Rabbit IgG Clone #014
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
2 μl/Test, 0.1 mg/ml
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow cytometric analysis of Mouse CD80 expression on LPS-stimulated BABL/c splenocytes. Cells were stained with FITC-conjugated anti-Mouse CD80. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD8, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD8 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.