Anti-CHEK2 Antibody

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Anti-CHEK2 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-CHEK2 Antibody
经验证的应用
WB,IHC-P,IP
交叉反应
Reacts with: Mouse
特异性
Mouse CHEK2
免疫原
Recombinant Mouse CHK2 / CHEK2 protein (Catalog#50432-M20B)
制备方法
Produced in rabbits immunized with purified, recombinant Mouse CHK2 / CHEK2 (rM CHK2 / CHEK2; Catalog#50432-M20B; Q9Z265; Met1-Leu546). CHK2 / CHEK2 specific IgG was purified by Mouse CHK2 / CHEK2 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CHEK2 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
IHC-P 1:2000-1:10000
IP 1-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CHEK2 Antibody (Rabbit Polyclonal 抗体) 图片

Immunochemical staining of mouse CHEK2 in mouse spleen with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.

Mouse CHEK2 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

0.5 µL anti-Mouse CHEK2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse CHEK2 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 61 kDa

Observed band size: 61 kDa

Anti-CHEK2 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hl-60 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:61 kDa

Observed band size:61 kDa

Anti-CHEK2 Antibody: 别称

Anti-Cds1 Antibody; Anti-CHK2 Antibody; Anti-HUCDS1 Antibody; Anti-Rad53 Antibody

CHEK2 背景信息

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
全称
checkpoint kinase 2
参考文献
  • Bogdanova N, et al. (2005) Association of two mutations in the CHEK2 gene with breast cancer. Cancer Genetics. 116(2) : 263-6.
  • Dong XY, et al. (2003) Mutations in CHEK2 associated with prostate cancer risk. The American journal of human genetics. 72(2) 270-80.
  • Massink MPG, Kooi IE, Martens JWM, Waisfisz Q, Meijers-Heijboer H. Genomic profiling of CHEK2*1100delC-mutated breast carcinomas. BMC Cancer. 2015;15:877. doi:10.1186/s12885-015-1880-y.
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