Anti-COX IV / COX4I1 Antibody

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Anti-COX IV / COX4I1 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-COX IV / COX4I1 Antibody
经验证的应用
WB,IHC-P,ICC/IF,IF
交叉反应
Reacts with: Human
特异性
Human COX IV / COX4I1
免疫原
A synthetic peptide corresponding to the C-terminus of the Human COX IV
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human COX IV, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-COX IV / COX4I1 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IHC-P 1:1000-1:5000
ICC-IF 1:300-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-COX IV / COX4I1 Antibody (Rabbit Polyclonal 抗体) 图片

Anti-COX4I1 rabbit polyclonal antibody at 1:500 dilution

Lane A: NIH3T3 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:15 kDa

Observed band size:17 kDa

(We are unsure as to the identity of these extra bands.)

Immunochemical staining of human COX4I1 in human liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human COX4I1 in human colon carcinoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of COX4I1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human COX4I1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Anti-COX IV / COX4I1 Antibody: 别称

Anti-COX IV-1 Antibody; Anti-COX4 Antibody; Anti-COX4-1 Antibody; Anti-COXIV Antibody; Anti-COXIV-1 Antibody
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