EGFR Protein, Mouse, Recombinant (His Tag)

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EGFR Protein, Mouse, Recombinant (His Tag): 产品信息

纯度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
1. Measured by its binding ability in a functional ELISA.
2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind  human EGF-Fc (Cat:10605-H01H), The EC50 of human EGF-Fc (Cat:10605-H01H) is 60-90 ng/mL.
3. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind  mouse EGF-Fc (Cat:50482-M01H), The EC50 of mouse EGF-Fc (Cat:50482-M01H) is 70-100 ng/mL.
蛋白构建
A DNA sequence encoding the extracellular domain of mouse EGFR (Q01279) (Met 1-Ser 647) was fused with a polyhistidine tag at the C-terminus.
NCBI Reference No.
表达宿主
HEK293 Cells
种属
Mouse
预测 N 端
Leu 25
分子量
The secreted recombinant mouse EGFR comprises 634 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, the apparent molecular mass of rmEGFR is approximately 100 kDa in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EGFR Protein, Mouse, Recombinant (His Tag): 图片

EGFR Protein, Mouse, Recombinant (His Tag): 别称

9030024J15Rik Protein, Mouse; AI552599 Protein, Mouse; Erbb Protein, Mouse; Errb1 Protein, Mouse; Errp Protein, Mouse; wa-2 Protein, Mouse; wa2 Protein, Mouse; Wa5 Protein, Mouse

EGFR 背景信息

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
全称
epidermal growth factor receptor
参考文献
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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